School of Pharmacy, Second Military Medical University, Shanghai, PR China.
J Ethnopharmacol. 2012 Jun 1;141(2):728-35. doi: 10.1016/j.jep.2011.08.011. Epub 2011 Aug 12.
Huang-Lian-Jie-Du-Tang (HLJDT), a classic prescription of traditional Chinese medicine (TCM), has been used in clinical over 1700 years for the treatment of gastrointestinal disorders, cardiovascular diseases and Alzheimer disease. But the active components of HLJDT were ambiguous, which seriously restricted its clinical application.
The methodology of plasma pharmacochemistry was applied to screen the bioactive components in HLJDT. A reliable LC/MS system was established for detecting the prototype compounds and metabolites in dosed plasma after oral administration of HLJDT. By comparative analysis of the chemical profiles of HLJDT extracts, blank plasma and dosed plasma, potential bioactive compounds in HLJDT may be discovered.
By comparing the retention time, MS and MS/MS spectra with those of reference standard and literature data, 30 components including 22 prototype compounds and 8 metabolites from HLJDT were discovered as potential bioactive components in rat plasma.
A reliable and effective method was established to screen the potential bioactive components in the formula of HLJDT, which provided useful information for the further study of action mechanism of HLJDT.
黄连解毒汤（HLJDT）是一种经典的中药方剂，已经在临床上使用了 1700 多年，用于治疗胃肠道疾病、心血管疾病和老年痴呆症。但 HLJDT 的活性成分不明确，这严重限制了其临床应用。
采用血浆药物化学方法筛选 HLJDT 中的生物活性成分。建立了一种可靠的 LC/MS 系统，用于检测口服 HLJDT 后给药血浆中的原型化合物和代谢物。通过比较 HLJDT 提取物、空白血浆和给药血浆的化学图谱，可以发现 HLJDT 中的潜在生物活性化合物。
通过比较保留时间、MS 和 MS/MS 图谱与对照品和文献数据，从 HLJDT 中发现了 30 种成分，包括 22 种原型化合物和 8 种代谢物，这些成分可能是 HLJDT 的潜在生物活性成分。
建立了一种可靠有效的方法来筛选 HLJDT 配方中的潜在生物活性成分，为进一步研究 HLJDT 的作用机制提供了有用的信息。
