School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200030, PR China.
J Pharm Biomed Anal. 2010 Nov 2;53(3):646-54. doi: 10.1016/j.jpba.2010.05.009. Epub 2010 May 13.
A plasma pharmacochemistry analysis of the bioactive constituents in rat plasma after oral administration of ChanSu was performed. High performance liquid chromatography coupled with the electrospray ionization mass spectrometry techniques of HPLC/ESI-IT-MS/MS and RRLC/ESI-Q-TOF-MS were used for the chemical profiling of samples of dosed plasma, control plasma and ChanSu extract. Comparative analysis of the resulting chemical profiles revealed 20 prototype compounds and 4 metabolites derived from ChanSu as potential bioactive components. By MS/MS analysis and accurate molecular weight assessments, the majority of these bioactive components (19 prototype compounds and 2 metabolites) were structurally identified. Moreover, seven were confirmed by comparing their retention behaviors using MS and MS/MS analysis. This study proposes a series of potential bioactive components of ChanSu, which we hope will lead to new drug discovery based on ChanSu and other traditional Chinese medicines.
采用高效液相色谱-电喷雾电离串联质谱（HPLC/ESI-IT-MS/MS）和串联四级杆飞行时间质谱（RRLC/ESI-Q-TOF-MS）联用技术对口服蟾酥后大鼠血浆中的生物活性成分进行了血浆药效化学分析。对给药后血浆、对照血浆和蟾酥提取物的样品进行了化学特征分析。比较分析结果表明，有 20 个原型化合物和 4 个代谢产物可能是蟾酥的潜在生物活性成分。通过 MS/MS 分析和精确分子量评估，大多数这些生物活性成分（19 个原型化合物和 2 个代谢产物）的结构得到了鉴定。此外，通过比较其保留行为，使用 MS 和 MS/MS 分析，确认了其中的 7 个。本研究提出了一系列蟾酥的潜在生物活性成分，我们希望这将为基于蟾酥和其他中药的新药发现提供依据。
