Department of Ophthalmology, A-Floor, Royal Hallamshire Hospital, Sheffield S10 2JF, Sheffield, UK.
Br J Ophthalmol. 2012 Mar;96(3):329-31. doi: 10.1136/bjophthalmol-2011-300245. Epub 2011 Aug 19.
To demonstrate a novel surgical technique for the accurate diagnosis of iris lesions using a minimally invasive aspiration cannula.
12 consecutive patients underwent biopsy of iris lesions at the Ocular Oncology Service, Royal Hallamshire Hospital, Sheffield, UK. Samples were obtained using a novel technique called trans-corneal fine cannula aspiration. This comprised a 25-gauge, Rycroft cannula aspiration technique performed by a single surgeon and samples transferred into alcohol-based tissue fixative. A specialist ophthalmic histopathologist performed a histological analysis of the samples.
On average, the size of the specimens obtained in theatre ranged from 1 mm to 1.5 mm (maximum dimensions). This sample size allowed an unequivocal histological diagnosis in all 12 cases. In this study, 10 patients were diagnosed as having iris melanoma, one patient with metastatic adenocarcinoma and one patient with pigmented adenoma.
This simple iris tumour biopsy technique provides sufficiently large sample sizes to obtain a firm histological diagnosis in 100% of cases performed so far. The sample sizes permitted not only morphological interpretation but also ancillary investigations such as immunohistochemistry.
展示一种使用微创抽吸套管准确诊断虹膜病变的新手术技术。
在英国谢菲尔德皇家哈利姆沙尔医院的眼肿瘤科服务中心，12 名连续患者接受了虹膜病变的活检。使用一种称为经角膜细套管抽吸的新技术获得样本。该技术由一位外科医生单独进行，包括使用 25 号 Rycroft 套管进行抽吸，并将样本转移到酒精基组织固定液中。一位专业的眼科病理学家对样本进行了组织学分析。
平均而言，在手术室获得的标本大小从 1 毫米到 1.5 毫米（最大尺寸）不等。在所有 12 例中，这种样本大小都能明确做出组织学诊断。在这项研究中，10 例患者被诊断为虹膜黑色素瘤，1 例患者为转移性腺癌，1 例患者为色素性腺瘤。
这种简单的虹膜肿瘤活检技术提供了足够大的样本量，迄今为止，已能在 100%的病例中获得明确的组织学诊断。样本量不仅允许进行形态学解释，还允许进行免疫组织化学等辅助检查。
