Babkov V V, Narvskaia O V
Antibiot Khimioter. 1990 Jan;35(1):26-7.
A procedure of bacteria application to disks from the colonies was used for determining antibiotic inactivation in the disks by the bacteria colonies after the disk direct contact with the colonies. Changes in the antibiotic activity in the disks were registered after incubation at 37 degrees C for 2 hours. It was shown that ampicillin resistant strains of E. coli K12 carrying R plasmids and strains of S. typhimurium and S. aureus inactivated the antibiotics in the disks and their population were homogenous in this respect. It is advisable to use the procedure in assaying drug resistance of bacterial populations.
采用将细菌接种到来自菌落的圆纸片上的方法，在圆纸片与菌落直接接触后，测定菌落对圆纸片中抗生素的灭活情况。在37℃孵育2小时后，记录圆纸片中抗生素活性的变化。结果表明，携带R质粒的大肠杆菌K12氨苄青霉素抗性菌株、鼠伤寒沙门氏菌菌株和金黄色葡萄球菌菌株可使圆纸片中的抗生素失活，且在这方面其群体具有同质性。该方法适用于检测细菌群体的耐药性。
