Departamento de Desarrollo de Tecnologías, Centro Interdisciplinario de Ciencias Marinas, Instituto Politécnico Nacional, La Paz, Mexico.
Eur Rev Med Pharmacol Sci. 2010 Sep;14(9):739-47.
Sixty ethanol extracts of marine flora of Baja California Sur (Mexico) were screened to evaluate the reversing effect of the bacterial resistance to antibiotics in combination with a sublethal concentration of ampicillin or erythromycin.
The activity was assayed by using a modification of the classical agar-diffusion method against 3 resistant, pathogenic bacteria; Escherichia coil (ATCC BAA196), Staphylococcus aureus (ATCC BAA42), and Streptococcus pyogenes (ATCC BAA946).
From the 60 ethanolic extracts, 12 (20%) of them in combination with ampicillin were able to reverse the resistance of Staphylococcus aureus and 8 (13%) with erythromycin yielded the same reversal with Streptococcus pyogenes. An extract from Sargassum horridum was the only one that reversed the resistance to antibiotics against both Staphylococcus aureus and Streptococcus pyogenes.
Our findings suggest that some algae may be source of compounds with the potential to reverse the antibiotic resistance of some bacteria. In addition, of the assayed extracts, 35 (57%) showed inhibitory activity against Staphylococcus aureus, 48 (78%) were active against Streptococcus pyogenes, but none was active against Escherichia coil. The most active extracts were from Laurencia spp., Gelidium robustum, Chnoospora implexa, Padina mexicana, Gracilaria subsecundata, and Dictyopteris undulata.
对下加利福尼亚州（墨西哥）的 60 种海洋植物的乙醇提取物进行筛选，以评估亚抑菌浓度氨苄青霉素或红霉素与抗生素联合使用对细菌耐药性的逆转作用。
采用改良的琼脂扩散法，对抗生素耐药的 3 种致病菌（大肠杆菌 ATCC BAA196、金黄色葡萄球菌 ATCC BAA42 和化脓性链球菌 ATCC BAA946）进行活性测定。
在 60 种醇提物中，有 12 种（20%）与氨苄青霉素联合使用可逆转金黄色葡萄球菌的耐药性，8 种（13%）与红霉素联合使用可使化脓性链球菌产生相同的逆转作用。来自 Sargassum horridum 的提取物是唯一一种能逆转金黄色葡萄球菌和化脓性链球菌对抗生素耐药性的物质。
我们的研究结果表明，一些藻类可能是具有逆转某些细菌抗生素耐药性的化合物的来源。此外，在所测试的提取物中，有 35 种（57%）对金黄色葡萄球菌具有抑制活性，48 种（78%）对化脓性链球菌具有活性，但对大肠杆菌均无活性。最具活性的提取物来自于 Laurencia spp.、 Gelidium robustum、 Chnoospora implexa、 Padina mexicana、 Gracilaria subsecundata 和 Dictyopteris undulata。
