从红球菌中提取糖脂生物表面活性剂的体外细胞因子刺激试验：单核细胞黏附的作用。
In vitro cytokine stimulation assay for glycolipid biosurfactant from Rhodococcus ruber: role of monocyte adhesion.
机构信息
Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, 13 Golev Street, 614081, Perm, Russia.
出版信息
Cytotechnology. 2011 Dec;63(6):559-66. doi: 10.1007/s10616-011-9384-3. Epub 2011 Aug 20.
Abstract
Glycolipid biosurfactant (GLB) from Rhodococcus ruber IEGM 231 was found to stimulate tumor necrosis factor-α (TNF-α), interleukin (IL) -1β and IL-6 production when applied as an ultrasonic emulsion to the adherent human peripheral blood monocyte culture. However, a lack of cytokine-stimulating activity was registered with the GLB applied as a hydrophobic film coating in 24-well culture plates, indicating that it may have been due to its inhibitory effect on monocyte adhesion. The mode of GLB application may therefore play an important role in in vitro assay of immunostimulatory activity of this compound as well as other bacterial glycolipids. Additionally, GLB from R. ruber displayed no cytotoxicity against human lymphocytes and therefore could be proposed as a potential immunomodulating and antitumor agent.
摘要
红平红球菌 IEGM 231 的糖脂生物表面活性剂（GLB）在作为超声乳液应用于贴壁人外周血单核细胞培养物时，被发现能刺激肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β和 IL-6 的产生。然而，当 GLB 作为疏水性薄膜涂层应用于 24 孔培养板时，并未记录到细胞因子刺激活性，这表明可能是由于其对单核细胞黏附的抑制作用。因此，GLB 的应用方式可能在该化合物以及其他细菌糖脂的体外免疫刺激活性测定中起着重要作用。此外，红平红球菌的 GLB 对人淋巴细胞没有细胞毒性，因此可以作为一种潜在的免疫调节和抗肿瘤药物。
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