从靶场中分离得到的蜡状芽孢杆菌对 2,4,6-三硝基苯酚的降解。
2,4,6-Trinitrophenol degradation by Bacillus cereus isolated from a firing range.
机构信息
Department of Biotechnology, Panjab University, Chandigarh 160014, India.
出版信息
Biotechnol Lett. 2011 Dec;33(12):2411-5. doi: 10.1007/s10529-011-0726-1. Epub 2011 Aug 21.
Bacillus cereus strain PU, isolated from soil contaminated with explosive waste, tolerated up to 1.3 mM 2,4,6 trinitrophenol (TNP) and utilize it aerobically as sole nitrogen and carbon source. Degradation of TNP was accompanied by stoichiometric release of 2.1 ± 0.15 mol nitrite/mol TNP at 539 μmol/h g dry cell wt. Metabolism of TNP was accompanied by transient accumulation of an orange-red metabolite, hydride meisenheimer complex (H-TNP), indicating a metabolic pathway involving complete reductive removal of the nitro group as nitrite.
从受爆炸物污染的土壤中分离到的蜡状芽孢杆菌 PU 菌株能够耐受高达 1.3mM 的 2,4,6-三硝基苯酚（TNP），并将其作为唯一的氮源和碳源进行好氧利用。TNP 的降解伴随着化学计量比为 2.1±0.15mol 亚硝酸盐/mol TNP 的亚硝酸的释放，其释放速率为 539μmol/h/g 干细胞重量。TNP 的代谢伴随着橙色-红色代谢物氢化物迈斯纳络合物（H-TNP）的短暂积累，表明存在一条代谢途径，涉及硝基基团作为亚硝酸盐的完全还原去除。
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