Shen Jinyou, Zhang Jianfa, Zuo Yi, Wang Lianjun, Sun Xiuyun, Li Jiansheng, Han Weiqing, He Rui
School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, Jiangsu Province, China.
J Hazard Mater. 2009 Apr 30;163(2-3):1199-206. doi: 10.1016/j.jhazmat.2008.07.086. Epub 2008 Jul 26.
A picric acid-degrading bacterium, strain NJUST16, was isolated from a soil contaminated by picric acid and identified as a member of Rhodococcus sp. based on 16S rRNA sequence. The degradation assays suggested that the strain NJUST16 could utilize picric acid as the sole source of carbon, nitrogen and energy. The isolate grew optimally at 30 degrees C and initial pH 7.0-7.5 in the mineral salts medium supplemented with picric acid. It was basically consistent with degradation of picric acid by the isolate. Addition of nitrogen sources such as yeast extract and peptone accelerated the degradation of picric acid. However, the stimulation was concentration dependent. The degradation was accompanied by release of stoichiometric amount of nitrite and acidification. The degradation of picric acid at relatively high concentrations (>3.93 mM) demonstrated that the degradation was both pH and nitrite dependent. Neutral and slightly basic pH was crucial to achieve high concentrations of picric acid degradation by the NJUST16 strain.
从被苦味酸污染的土壤中分离出一株苦味酸降解菌NJUST16，基于16S rRNA序列鉴定其为红球菌属的一员。降解试验表明，菌株NJUST16能够利用苦味酸作为唯一的碳、氮和能量来源。该分离株在添加了苦味酸的矿物盐培养基中，于30℃和初始pH 7.0 - 7.5条件下生长最佳。这与该分离株对苦味酸的降解基本一致。添加酵母提取物和蛋白胨等氮源可加速苦味酸的降解。然而，这种促进作用是浓度依赖性的。降解过程伴随着化学计量的亚硝酸盐释放和酸化。在相对高浓度（>3.93 mM）下苦味酸的降解表明，降解既依赖于pH值，也依赖于亚硝酸盐。中性和略碱性pH对于NJUST16菌株实现高浓度苦味酸降解至关重要。
