The Faculty of Sciences and Letters, Department of Molecular Biology and Genetics, Kilis 7 Aralık University, 79000, Kilis, Turkey,
Microb Ecol. 2013 Oct;66(3):512-21. doi: 10.1007/s00248-013-0248-6. Epub 2013 May 29.
In this study, biological degradation of 2,4,6-trinitrotoluene (TNT) which is very highly toxic environmentally and an explosive in nitroaromatic character was researched in minimal medium by Bacillus cereus isolated from North Atlantic Treaty Organization (NATO) TNT-contaminated soils. In contrast to most previous studies, the capability of this bacteria to transform in liquid medium containing TNT was investigated. During degradation, treatment of TNT was followed by high-performance liquid chromatography (HPLC) and achievement of degradation was calculated as percentage. At an initial concentration of 50 and 75 mg L(-1), TNT was degraded respectively 68 % and 77 % in 96 h. It transformed into 2,4-dinitrotoluene and 4-aminodinitrotoluene derivates, which could be detected as intermediate metabolites by using thin-layer chromatography and gas chromatography-mass spectrometry analyses. Release of nitrite and nitrate ions were searched by spectrophotometric analyses. Depending upon Meisenheimer complex, while nitrite production was observed, nitrate was detected in none of the cultures. Results of our study propose which environmental pollutant can be removed by using microorganisms that are indigenous to the contaminated site.
本研究采用从北大西洋公约组织（北约）受 TNT 污染土壤中分离出的蜡状芽孢杆菌，在最小培养基中研究了具有高度环境毒性和硝基芳香族爆炸物特征的 2,4,6-三硝基甲苯（TNT）的生物降解。与大多数先前的研究不同，本研究调查了该细菌在含有 TNT 的液体培养基中的转化能力。在降解过程中，采用高效液相色谱（HPLC）跟踪 TNT 的处理情况，并计算降解率。在初始浓度为 50 和 75mg/L 时，TNT 在 96 小时内分别降解了 68%和 77%。它转化为 2,4-二硝基甲苯和 4-氨基二硝基甲苯衍生物，可通过薄层层析和气相色谱-质谱分析检测到这些中间代谢产物。通过分光光度分析寻找亚硝酸盐和硝酸盐离子的释放。根据迈森海默络合物，虽然观察到亚硝酸盐的产生，但在任何培养物中都未检测到硝酸盐。本研究结果表明，可以利用污染场地土著微生物去除环境污染物。
