Simon-Assmann Patricia, Orend Gertraud, Mammadova-Bach Elmina, Spenlé Caroline, Lefebvre Olivier
Université de Strasbourg, Strasbourg, France.
Int J Dev Biol. 2011;55(4-5):455-65. doi: 10.1387/ijdb.103223ps.
The interaction of endothelial cells and pericytes with their microenvironment, in particular with the basement membrane, plays a crucial role during vasculogenesis and angiogenesis. In this review, we focus on laminins, a major family of extracellular matrix molecules present in basement membranes. Laminins interact with cell surface receptors to trigger intracellular signalling that shapes cell behaviour. Each laminin exerts a distinct effect on endothelial cells and pericytes which largely depends on the adhesion receptor profile expressed on the cell surface. Moreover, proteolytic cleavage of laminins may affect their role in angiogenesis. We report in vitro and in vivo data on laminin-111, -411, -511 and -332 and their associated signalling that regulates cell behaviour and angiogenesis under normal and pathological conditions. We also discuss how tissue-specific deletion of laminin genes affects the behaviour of endothelial cells and pericytes and thus angiogenesis. Finally, we examine how coculture systems with defined laminin expression contribute to our understanding of the roles of laminins in normal and pathological vasculogenesis and angiogenesis.
内皮细胞和平周细胞与其微环境，特别是与基底膜的相互作用，在血管发生和血管生成过程中起着关键作用。在本综述中，我们聚焦于层粘连蛋白，它是基底膜中存在的细胞外基质分子的一个主要家族。层粘连蛋白与细胞表面受体相互作用，触发塑造细胞行为的细胞内信号传导。每种层粘连蛋白对内皮细胞和平周细胞都有独特的影响，这在很大程度上取决于细胞表面表达的黏附受体谱。此外，层粘连蛋白的蛋白水解切割可能会影响它们在血管生成中的作用。我们报告了关于层粘连蛋白-111、-411、-511和-332及其相关信号传导的体外和体内数据，这些信号传导在正常和病理条件下调节细胞行为和血管生成。我们还讨论了层粘连蛋白基因的组织特异性缺失如何影响内皮细胞和平周细胞的行为，进而影响血管生成。最后，我们研究了具有特定层粘连蛋白表达的共培养系统如何有助于我们理解层粘连蛋白在正常和病理血管发生及血管生成中的作用。
