School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.
Nat Prod Res. 2012;26(3):255-63. doi: 10.1080/14786419.2010.537272. Epub 2011 Aug 23.
Historically, Schisandra chinensis and S. sphenanthera have been widely used in traditional Chinese medicine. Although both species are in the genus Schisandra, they have dissimilar therapeutic effects that may be attributed to compositional differences in secondary metabolites. We developed a method to compare these metabolites obtained from the above plant species using ultra-performance liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry coupled with multivariate statistical analyses. The critical markers we used to discriminate between both plant species resulted in the identification and quantification of six lignans and seven essential oils. We believe that our approach provided a sensitive, reliable and robust method to conveniently classify medicinal plants that can be used to explore subtle variations among different species or plants from different geographical locations.
从历史上看，五味子和华中五味子在中医药中被广泛应用。尽管这两个物种都属于五味子属，但它们具有不同的治疗效果，这可能归因于次生代谢产物成分的差异。我们开发了一种使用超高效液相色谱-质谱联用和气相色谱-质谱联用结合多变量统计分析来比较这两种植物来源的代谢物的方法。我们用于区分这两个植物物种的关键标志物导致了六种木脂素和七种精油的鉴定和定量。我们相信，我们的方法提供了一种敏感、可靠和强大的方法，可以方便地对药用植物进行分类，用于探索不同物种或不同地理位置的植物之间的细微差异。
