College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
Food Chem. 2013 Dec 15;141(4):3931-7. doi: 10.1016/j.foodchem.2013.06.064. Epub 2013 Jun 24.
Discrimination of the origins of plants as traditional medicinal herbs or functional foods is important to accurately comprehend their therapeutic effects or to appropriately utilize their qualities because different environmental backgrounds can induce diverse metabolic changes. In the present study, the origins of the herbal medicine Schisandra chinensis were differentiated using two instrumental approaches, GC/MS and LC/MS. The acquired data were processed using various programs to detect metabolites and statistically examined to measure the suitability of the methods. The R(2)X value of the PCA analysis was used to examine the identified metabolites as potential discriminative markers. The identification of markers by primary metabolites using GC/MS analysis was advantageous because of its reproducibility and the use of a constructed database. However, LC/MS analysis using secondary metabolites provided a greater number of distinguishable variables and higher qualitative R(2)X values for the markers, which suggested that determination of the origins of the plants was more favourable using secondary metabolites.
鉴别植物作为传统草药或功能性食品的起源对于准确理解其治疗效果或适当利用其品质非常重要，因为不同的环境背景会引起不同的代谢变化。在本研究中，使用两种仪器方法（GC/MS 和 LC/MS）对草药五味子的起源进行了区分。使用各种程序处理获得的数据，并进行了统计学检查以衡量方法的适用性。主成分分析（PCA）的 R(2)X 值用于检查所鉴定的代谢物作为潜在的鉴别标记。使用 GC/MS 分析对初级代谢物进行标记物的鉴定具有优势，因为其具有可重复性和使用构建的数据库。然而，使用二级代谢物的 LC/MS 分析提供了更多可区分的变量和更高的定性 R(2)X 值，这表明使用二级代谢物更有利于确定植物的起源。
