α-银环蛇毒素在用氯胺-T进行放射性碘化时会聚集，但用碘珠法时则不会。
alpha-Bungarotoxin aggregates on radioiodination with chloramine-T but not with iodogen.
作者信息
Jailkhani B L, Asthana D, Jaffery N F, Subbalaxmi B
出版信息
J Neuroimmunol. 1984 Aug;6(5):337-45. doi: 10.1016/0165-5728(84)90023-7.
The presence of polymer(s) of radioiodinated bungarotoxin (Bgt) in preparations iodinated with chloramine-T or with iodogen, was investigated by chromatography on Sephadex-G50. In addition to a monomeric peak (P2), chloramine-T preparations showed a high content of polymeric forms (16-43%) eluting in the void volume (P1), present only to the extent of 1-3% in iodogen preparations. In purified [125I]Bgt prepared by the iodogen method, the content of P1 increased on treatment with chloramine-T, in the absence of radioiodine. The nonspecific binding of [125I]Bgt to DE-81 filter discs was high in the case of chloramine-T preparations and proportional to the content of P1 in each batch. Purified P1 (polymer) but not P2 (monomer) showed a concentration-dependent high degree of binding to DE-81 discs.
通过在葡聚糖凝胶G-50上进行色谱分析，研究了用氯胺-T或碘代甘氨酸碘化的制剂中放射性碘化银环蛇毒素（Bgt）聚合物的存在情况。除了单体峰（P2）外，氯胺-T制剂在空体积（P1）中洗脱时显示出高含量的聚合形式（16 - 43%），而碘代甘氨酸制剂中仅占1 - 3%。在用碘代甘氨酸法制备的纯化[125I]Bgt中，在无放射性碘的情况下用氯胺-T处理后，P1的含量增加。氯胺-T制剂中[125I]Bgt与DE-81滤膜的非特异性结合很高，且与每批中P1的含量成正比。纯化的P1（聚合物）而非P2（单体）显示出与DE-81滤膜的浓度依赖性高度结合。
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