Adeli K, Sinkevitch C
Department of Chemistry and Biochemistry, University of Windsor, Ontario, Canada.
FEBS Lett. 1990 Apr 24;263(2):345-8. doi: 10.1016/0014-5793(90)81410-p.
We have developed a defined medium which can maintain efficient growth of HepG2 cells sustaining the synthesis of a variety of plasma proteins including apolipoprotein B. This defined system was used to investigate long-term effects of insulin, estrogen, triiodothyronine, cholesterol, and oleate on the growth pattern of HepG2 cells and secretion rate of apolipoprotein B. Oleate and triiodothyronine caused significant increases in secretion of apolipoprotein B. The stimulatory effect of triiodothyronine was only observed after long (6 days) exposure of cells to the hormone. In contrast, insulin caused up to a 4-fold decrease in the secretion rate of apolipoprotein B during the early growth periods. This inhibitory effect appeared to be partially abolished after 6 days. Our data suggest that some important questions on regulation of apolipoprotein B expression can be addressed by the long-term culture of HepG2 cells in defined medium.
我们已经开发出一种限定培养基，它能够维持HepG2细胞的高效生长，并持续合成包括载脂蛋白B在内的多种血浆蛋白。这个限定系统被用于研究胰岛素、雌激素、三碘甲状腺原氨酸、胆固醇和油酸对HepG2细胞生长模式和载脂蛋白B分泌率的长期影响。油酸和三碘甲状腺原氨酸导致载脂蛋白B的分泌显著增加。三碘甲状腺原氨酸的刺激作用只有在细胞长期（6天）暴露于该激素后才会观察到。相比之下，胰岛素在早期生长阶段使载脂蛋白B的分泌率降低了4倍。这种抑制作用在6天后似乎部分被消除。我们的数据表明，通过在限定培养基中对HepG2细胞进行长期培养，可以解决一些关于载脂蛋白B表达调控的重要问题。
