Kosykh V A, Preobrazhensky S N, Fuki I V, Zaikina O E, Tsibulsky V P, Repin V S, Smirnov V N
Biochim Biophys Acta. 1985 Oct 2;836(3):385-9. doi: 10.1016/0005-2760(85)90143-2.
During a 5 day cultivation of human hepatocytes in a primary culture the secretion of apolipoprotein B was measured by enzyme-linked immunosorbent assay. Density-gradient ultracentrifugation demonstrated that the majority of the secreted apolipoprotein B was associated with the very-low-density lipoprotein fraction. Exposure of the cells to cholesterol (5-100 micrograms/ml) resulted in a dose-dependent increase in apolipoprotein B secretion rate.
在原代培养中对人肝细胞进行5天培养期间，通过酶联免疫吸附测定法测量载脂蛋白B的分泌。密度梯度超速离心表明，分泌的载脂蛋白B大部分与极低密度脂蛋白部分相关。将细胞暴露于胆固醇（5 - 100微克/毫升）会导致载脂蛋白B分泌率呈剂量依赖性增加。
