Kanter Ahmet, Celik Ismail
Department of Biology, Yuzuncu Yil University, Van, Turkey.
Toxicol Ind Health. 2012 May;28(4):369-76. doi: 10.1177/0748233711412426. Epub 2011 Aug 22.
This study was aimed mainly to assess the effects of fenthion on certain oxidative stress biomarkers in various tissues of frogs (Rana ridibunda). Biomarkers selected for stress monitoring were malondialdehyde (MDA) and antioxidant defense system (ADS) such as reduced glutathione (GSH) level, glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), and superoxide dismutase (SOD) activities in the liver, kidney, heart, and brain of frogs exposed to 10 and 20 ppm dosages of fenthion for 24, 48, 72, and 96 h. The results demonstrate an increase in MDA levels in selected tissues following exposure to both concentrations of fenthion. The ADS, GSH-Px, GST, SOD activities and GSH levels also fluctuated after 24, 48, 72, and 96 h in all the treatment groups compared with controls. From the evidence obtained here, it is concluded that the exposure of frogs to fenthion induced an increase in MDA combined with fluctuated ADS. This may reflect the potential role of these parameters as useful biomarkers for oxidative stress in amphibian species.
本研究主要旨在评估倍硫磷对青蛙（泽蛙）各组织中某些氧化应激生物标志物的影响。用于应激监测的生物标志物包括丙二醛（MDA）以及抗氧化防御系统（ADS），如暴露于10 ppm和20 ppm倍硫磷剂量24、48、72和96小时的青蛙肝脏、肾脏、心脏和大脑中的还原型谷胱甘肽（GSH）水平、谷胱甘肽过氧化物酶（GSH-Px）、谷胱甘肽-S-转移酶（GST）和超氧化物歧化酶（SOD）活性。结果表明，暴露于两种浓度的倍硫磷后，所选组织中的MDA水平均有所升高。与对照组相比，所有处理组在24、48、72和96小时后，ADS、GSH-Px、GST、SOD活性以及GSH水平也出现了波动。根据此处获得的证据，得出的结论是，青蛙暴露于倍硫磷会导致MDA增加，同时ADS波动。这可能反映了这些参数作为两栖动物氧化应激有用生物标志物的潜在作用。
