Celik Ismail, Isik Ismail, Ozok Necati, Salih Kaya M
Department of Biology, Faculty of Science and Letters, Yuzuncu Yil University, Van, Turkey.
Toxicol Ind Health. 2011 May;27(4):357-62. doi: 10.1177/0748233710387009. Epub 2010 Oct 14.
The study was carried out to investigate the neurotoxic and immunotoxic effects of fenthion- and omethoate-used agricultural areas on frogs (Rana ridibunda) at acute exposure. The neurotoxic effects of the chemicals were evaluated by measuring the activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Biomarkers selected for immunotoxic monitoring were the activities of adenosine deaminase (ADA) and myeloperoxidase (MPO) in various tissues of frogs exposed to 10 or 20 parts per million (ppm) dosages of fenthion and omethoate for 24, 48, 72 and 96 hours. Results showed that the administrations of chemicals fluctuated AChE and BChE activities in some tissues of frogs treated with both dosages at all the periods. With regard to the immunotoxic effects, MPO activity was increased in almost all the tissues of frogs after 10 and 20 ppm dosages and for 24, 48, 72 and 96 hours exposure of fenthion and omethoate as compared to those of control whereas ADA activity did not change in all the tissues. This may reflect the potential role of these parameters as useful biomarkers for toxicity of fenthion and omethoate.
本研究旨在调查急性暴露下，使用倍硫磷和氧乐果的农业区域对青蛙（泽蛙）的神经毒性和免疫毒性作用。通过测量乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）的活性来评估这些化学物质的神经毒性作用。选择用于免疫毒性监测的生物标志物是暴露于百万分之10或20（ppm）剂量的倍硫磷和氧乐果24、48、72和96小时的青蛙各组织中腺苷脱氨酶（ADA）和髓过氧化物酶（MPO）的活性。结果表明，在所有时间段，两种剂量处理的青蛙的某些组织中，化学物质的施用使AChE和BChE活性发生波动。关于免疫毒性作用，与对照组相比，在暴露于10和20 ppm剂量的倍硫磷和氧乐果24、48、72和96小时后，青蛙几乎所有组织中的MPO活性均增加，而所有组织中的ADA活性均未改变。这可能反映了这些参数作为倍硫磷和氧乐果毒性有用生物标志物的潜在作用。
