Leibniz Institute of Plant Biochemistry, Stress and Developmental Biology, Weinberg 3, D-06120 Halle, Germany.
Mol Plant. 2012 Jan;5(1):115-30. doi: 10.1093/mp/ssr064. Epub 2011 Aug 22.
Calcium acts as a second messenger for signaling to a variety of stimuli including MAMPs (Microbe-Associated Molecular Patterns), such as flg22 and elf18 that are derived from bacterial flagellin and elongation factor Tu, respectively. Here, Arabidopsis thaliana mutants with changed calcium elevation (cce) in response to flg22 treatment were isolated and characterized. Besides novel mutant alleles of the flg22 receptor, FLS2 (Flagellin-Sensitive 2), and the receptor-associated kinase, BAK1 (Brassinosteroid receptor 1-Associated Kinase 1), the new cce mutants can be categorized into two main groups-those with a reduced or an enhanced calcium elevation. Moreover, cce mutants from both groups show differential phenotypes to different sets of MAMPs. Thus, these mutants will facilitate the discovery of novel components in early MAMP signaling and bridge the gaps in current knowledge of calcium signaling during plant-microbe interactions. Last but not least, the screening method is optimized for speed (covering 384 plants in 3 or 10 h) and can be adapted to genetically dissect any other stimuli that induce a change in calcium levels.
钙作为第二信使，可将多种刺激（包括源自细菌鞭毛蛋白和延伸因子 Tu 的 MAMP（微生物相关分子模式），如 flg22 和 elf18）的信号传递至细胞内。在此，我们分离并鉴定了对 flg22 处理反应中钙升高（cce）改变的拟南芥突变体。除了 flg22 受体 FLS2（Flagellin-Sensitive 2）和受体相关激酶 BAK1（Brassinosteroid receptor 1-Associated Kinase 1）的新型突变等位基因外，新的 cce 突变体还可分为两组——钙升高减少或增强的突变体。此外，两组中的 cce 突变体对不同的 MAMP 表现出不同的表型。因此，这些突变体将有助于发现早期 MAMP 信号转导中的新成分，并弥合当前植物-微生物相互作用中钙信号转导知识的空白。最后但同样重要的是，筛选方法速度得到优化（在 3 或 10 小时内覆盖 384 株植物），并且可以适应于遗传解析任何其他引起钙水平变化的刺激。
