Department of Plant Science, Faculty of Agricultural and Food Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Int J Mol Sci. 2021 May 1;22(9):4812. doi: 10.3390/ijms22094812.
An oxidative burst is an early response of plants to various biotic/abiotic stresses. In plant-microbe interactions, the plant body can induce oxidative burst to activate various defense mechanisms to combat phytopathogens. A localized oxidative burst is also one of the typical behaviors during hypersensitive response (HR) caused by gene-for-gene interaction. In this study, the occurrence of oxidative burst and its signaling pathways was studied from different levels of disease severity (i.e., susceptible, intermediate, and resistant) in the - pathosystem. Canola cotyledons with distinct levels of resistance exhibited differential regulation of the genes involved in reactive oxygen species (ROS) accumulation and responses. Histochemical assays were carried out to understand the patterns of HO accumulation and cell death. Intermediate and resistant genotypes exhibited earlier accumulation of HO and emergence of cell death around the inoculation origins. The observations also suggested that the cotyledons with stronger resistance were able to form a protective region of intensive oxidative bursts between the areas with and without hyphal intrusions to block further fungal advancement to the uninfected regions. The qPCR analysis suggested that different onset patterns of some marker genes in ROS accumulation/programmed cell death (PCD) such as , were associated with distinct levels of resistance from cultivars against . The observations and datasets from this article indicated the distinct differences in ROS-related cellular behaviors and signaling between compatible and incompatible interactions.
氧化爆发是植物对各种生物/非生物胁迫的早期反应。在植物-微生物相互作用中，植物体可以诱导氧化爆发，激活各种防御机制来抵御植物病原体。局部氧化爆发也是基因对基因相互作用引起的过敏反应 (HR) 的典型行为之一。在这项研究中，从易感性、中间性和抗性等不同严重程度水平研究了 - 系统中氧化爆发的发生及其信号通路。具有不同抗性水平的油菜子叶表现出与活性氧 (ROS) 积累和反应相关基因的差异调节。进行组织化学分析以了解 HO 积累和细胞死亡的模式。中间和抗性基因型表现出更早的 HO 积累和细胞死亡出现在接种起源周围。这些观察结果还表明，具有更强抗性的子叶能够在有和没有菌丝入侵的区域之间形成一个强烈氧化爆发的保护区域，以阻止真菌进一步向未感染区域的扩展。qPCR 分析表明，ROS 积累/编程性细胞死亡 (PCD) 中一些标记基因的不同起始模式，如 ，与 品种对 的不同抗性水平有关。本文的观察和数据集表明，在相容和不相容相互作用中，ROS 相关的细胞行为和信号存在明显差异。
