Qin Fenju, Zhang Jie, Wang Taoyun, Tong Jian
Department of Chemistry and Bioengineering, Suzhou University of Science and Technology, Suzhou 215009, China.
Wei Sheng Yan Jiu. 2011 Jul;40(4):499-502.
To study the effect and mechanism of Soybean Isoflavones (SIF) on the cognitive performance of female mice after sleep deprivation for 72h.
Forty female mice were randomly divided into 5 groups:the control, sleep deprivation control (SDC), and three SIF groups (SIF L, SIF M and SIF H). SIF groups were administered by intra-gastric injection of SIF 20, 40 and 80 mg/kg bw respectively for 30 days. The control and SDC groups were administered with equal volume of normal saline. The 72h SD mice model was induced by the single platform technique. The cognitive ability of mice was tested by Y-maze. Antioxidant indicators (CAT, MDA and T-AOC) and neurotransmitters (NO, Glu and Ach) in cerebra were measured.
Compared with the control group, the cognitive abilities, CAT activities, T-AOC and Ach levels were decreased and NO, MDA and GLu contents were increased in mice after sleep deprivation for 72h. Compared with the SDC group, the cognitive abilities were improved and Ach contents were increased in SIF M and SIF H groups, T-AOC levels were improved in three SIF groups. CAT activities, MDA, NO and Glu contents were decreased in SIF H group only.
The cognitive ability of 72h SD female mice could be improved by SIF, the mechanism of which might be involved in increasing antioxidant function, decreasing the damage of free radicals on cranial nerves and adjusting the neuron transmitter of cerebra.
研究大豆异黄酮（SIF）对睡眠剥夺72小时后雌性小鼠认知能力的影响及机制。
将40只雌性小鼠随机分为5组：对照组、睡眠剥夺对照组（SDC）和3个SIF组（SIF L、SIF M和SIF H）。SIF组分别通过胃内注射20、40和80 mg/kg体重的SIF，持续30天。对照组和SDC组给予等量生理盐水。采用单平台技术诱导建立72小时睡眠剥夺小鼠模型。通过Y迷宫测试小鼠的认知能力。检测大脑中的抗氧化指标（CAT、MDA和T-AOC）和神经递质（NO、Glu和Ach）。
与对照组相比，睡眠剥夺72小时后的小鼠认知能力、CAT活性、T-AOC和Ach水平降低，NO、MDA和GLu含量增加。与SDC组相比，SIF M组和SIF H组的认知能力提高，Ach含量增加，3个SIF组的T-AOC水平提高。仅SIF H组的CAT活性、MDA、NO和Glu含量降低。
SIF可提高72小时睡眠剥夺雌性小鼠的认知能力，其机制可能与增强抗氧化功能、减少自由基对脑神经的损伤以及调节大脑神经递质有关。
