The W Harry Feinstone Department of Molecular Microbiology & Immunology, and, Johns Hopkins Malaria Research Institute, Bloomberg School of Public Health, Baltimore, MD 21205, USA.
Future Microbiol. 2011 Aug;6(8):851-3. doi: 10.2217/fmb.11.71.
Fang W, Vega-Rodríguez J, Ghosh AK et al. Development of transgenic fungi that kill human malaria parasites in mosquitoes. Science 331(6020), 1074-1077 (2011). Paratransgenesis is the genetic manipulation of insect endosymbiotic microorganisms such as bacteria, viruses or fungi. Paratransgenesis has been proposed as a potential method to control vector-borne diseases such as malaria. In this article, Fang and colleagues have used genetic manipulation to insert multiple antimalaria effector genes into the entomopathogenic fungus Metarhizium anisopliae. When the modified fungus was used to infect Anopheles mosquitoes, it expressed the antimalaria effector molecules in the mosquito hemolymph. When several different effector molecules were coexpressed, malaria levels in the mosquito salivary glands were inhibited by up to 98% compared with controls. Significant inhibition could be initiated by as little as seven fungal spores and was very rapid and long lasting. These data suggest that recombinant entomopathogenic fungi could be deployed as part of a strategy to control malaria.
方维，维加 - 罗德里格斯，阿克什等人。转基因真菌的开发，可在蚊子中杀死人类疟疾寄生虫。科学 331（6020），1074-1077（2011）。共生体基因转移是对昆虫共生微生物（如细菌、病毒或真菌）的遗传操纵。共生体基因转移已被提议作为控制疟疾等媒介传播疾病的潜在方法。在本文中，方等人使用遗传操作将多个抗疟效应基因插入昆虫病原真菌金龟子绿僵菌中。当修饰后的真菌用于感染按蚊时，它在蚊子血淋巴中表达抗疟效应分子。当几种不同的效应分子共表达时，与对照相比，蚊子唾液腺中的疟疾水平抑制了高达 98%。只需七个真菌孢子就可以引发显著的抑制作用，并且非常迅速和持久。这些数据表明，重组昆虫病原真菌可以作为控制疟疾策略的一部分进行部署。
