Karami Mohsen, Moosa-Kazemi Seyed Hassan, Oshaghi Mohammad Ali, Vatandoost Hasan, Sedaghat Mohammad Mehdi, Rajabnia Ramazan, Hosseini Mostafa, Maleki-Ravasan Naseh, Yahyapour Yousef, Ferdosi-Shahandashti Elaheh
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Infectious Diseases & Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran.
J Arthropod Borne Dis. 2016 Jan 6;10(3):347-63. eCollection 2016 Sep.
Wolbachia are common intracellular bacteria that infect different groups of arthropods including mosquitoes. These bacteria modify host biology and may induce feminization, parthenogenesis, male killing and cytoplasmic incompatibility (CI). Recently Wolbachia is being nominated as a bio-agent and paratransgenic candidate to control mosquito borne diseases.
Here we report the results of a survey for presence, frequency, and phylogenetic congruence of these endosymbiont bacteria in Culex pipiens populations in Northern, Central, and Southern parts of Iran using nested-PCR amplification of wsp gene.
Wolbachia DNA were found in 227 (87.3%) out of 260 wild-caught mosquitoes. The rate of infection in adult females ranged from 61.5% to 100%, while in males were from 80% to 100%. The Blast search and phylogenetic analysis of the wsp gene sequence revealed that the Wolbachia strain from Iranian Cx. pipiens was identical to the Wolbachia strains of supergroup B previously reported in members of the Cx. pipiens complex. They had also identical sequence homology with the Wolbachia strains from a group of distinct arthropods including lepidopteran, wasps, flies, damselfly, thrips, and mites from remote geographical areas of the world.
It is suggested that Wolbachia strains horizontally transfer between unrelated host organisms over evolutionary time. Also results of this study indicates that Wolbachia infections were highly prevalent infecting all Cx. pipiens populations throughout the country, however further study needs to define Wolbachia inter-population reproductive incompatibility pattern and its usefulness as a bio-agent control measure.
沃尔巴克氏体是常见的细胞内细菌，可感染包括蚊子在内的不同节肢动物群体。这些细菌会改变宿主生物学特性，并可能诱发雌性化、孤雌生殖、雄性致死和细胞质不亲和（CI）。最近，沃尔巴克氏体被提名为控制蚊媒疾病的生物制剂和拟转基因候选物。
在此，我们报告了一项调查结果，该调查使用 wsp 基因的巢式 PCR 扩增，来检测伊朗北部、中部和南部尖音库蚊种群中这些内共生细菌的存在情况、频率和系统发育一致性。
在 260 只野生捕获的蚊子中，有 227 只（87.3%）检测到沃尔巴克氏体 DNA。成年雌性的感染率在 61.5%至 100%之间，而雄性的感染率在 80%至 100%之间。对 wsp 基因序列的 Blast 搜索和系统发育分析表明，来自伊朗尖音库蚊的沃尔巴克氏体菌株与先前在尖音库蚊复合体成员中报道的 B 超群沃尔巴克氏体菌株相同。它们与来自世界偏远地区的一组不同节肢动物（包括鳞翅目、黄蜂、苍蝇、豆娘、蓟马和螨虫）的沃尔巴克氏体菌株也具有相同的序列同源性。
表明沃尔巴克氏体菌株在进化过程中在不相关的宿主生物体之间进行水平转移。此外，本研究结果表明，沃尔巴克氏体感染在全国所有尖音库蚊种群中高度流行，然而，需要进一步研究来确定沃尔巴克氏体种群间的生殖不亲和模式及其作为生物制剂控制措施的有效性。
