Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands.
Future Microbiol. 2011 Aug;6(8):941-51. doi: 10.2217/fmb.11.72.
The walls of many fungal species consist of a polysaccharide network offering mechanical strength and functioning as a scaffold for covalently attached glycoproteins. The rapid advances in fungal genome sequencing and mass spectrometry have made it possible to study fungal wall proteomes in detail, both qualitatively and quantitatively. One of the surprising outcomes of these studies is the large variety of covalently attached proteins found in fungal walls. Another important result is that fungi can rapidly adapt the protein composition of their new walls to changes in environmental conditions. The wall proteome of the opportunistic human pathogen Candida albicans amply illustrates these properties. Finally, we discuss the relevance of our insights for the identification of new vaccine candidates.
许多真菌物种的细胞壁由多糖网络组成，提供机械强度，并作为共价连接的糖蛋白的支架。真菌基因组测序和质谱技术的快速发展使得对真菌细胞壁的蛋白质组进行定性和定量研究成为可能。这些研究的一个令人惊讶的结果是，在真菌细胞壁中发现了大量共价连接的蛋白质。另一个重要的结果是，真菌可以迅速使新细胞壁的蛋白质组成适应环境条件的变化。机会性病原体白念珠菌的细胞壁蛋白质组充分说明了这些特性。最后，我们讨论了我们的见解对于鉴定新的疫苗候选物的相关性。
