School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, and Department of Psychiatry, Kaohsiung Kai-Suan Psychiatric Hospital, Kaohsiung 807, Taiwan.
J Chromatogr A. 2011 Sep 23;1218(38):6832-7. doi: 10.1016/j.chroma.2011.07.086. Epub 2011 Aug 10.
Methadone (MET) metabolism has been largely demonstrated with a high inter-individual variability and, therefore, quantification of MET is very important for therapeutic drug monitoring. A cation-selective exhaustive injection and sweeping MEKC (CSEI-Sweeping) was first developed to analyze MET and its two metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline (EMDP), in human serum. After pretreatment, the samples were electrokinetically injected into capillary (10 kV, 500s) and swept by the separation phosphate buffer (100 mM, pH 4.0) containing 20% tetrahydrofuran and 100 mM SDS at -15 kV. The LODs were 200 pg/mL for MET and EMDP, and 400 pg/mL for EDDP. Ten volunteers were administered MET (5.0-120.0 mg/day) orally for 84 days and serum samples were taken after the daily dose of MET (days 1, 2, 7, 14, 28, 56 and 84) individually. This method was used for monitoring MET and its metabolites in heroin addicts and for pharmacokinetic investigations.
美沙酮（MET）的代谢已被大量证明存在个体间的高度变异性，因此，对 MET 的定量分析对于治疗药物监测非常重要。首次开发了阳离子选择性耗尽进样和扫集 MEKC（CSEI-Sweeping）来分析人血清中的 MET 及其两种代谢物，2-亚乙基-1,5-二甲基-3,3-二苯基吡咯烷（EDDP）和 2-乙基-5-甲基-3,3-二苯基-1-吡咯啉（EMDP）。预处理后，样品以 10 kV 进行电动进样（500s），并用含有 20%四氢呋喃和 100 mM SDS 的分离磷酸盐缓冲液（100 mM，pH 4.0）在-15 kV 下进行扫集。MET 和 EMDP 的检出限为 200 pg/mL，EDDP 的检出限为 400 pg/mL。10 名志愿者每天口服美沙酮（5.0-120.0 mg/天），共 84 天，分别在服用 MET 后的第 1、2、7、14、28、56 和 84 天采集血清样本。该方法用于监测海洛因成瘾者的美沙酮及其代谢物，并进行药代动力学研究。
