Department of Chemical Sciences, Tezpur University, Napaam, Sonitpur-784028, Assam, India.
Ultrason Sonochem. 2012 Mar;19(2):292-9. doi: 10.1016/j.ultsonch.2011.07.010. Epub 2011 Jul 31.
Lycopene is bequeathed with multiple bio-protective roles, primarily attributed to its unique molecular structure. The concomitant exploitation of two of the green chemistry tools viz., sonication and biocatalysis is reported here for the laboratory scale extraction of lycopene from tomato peel. The coupled system improved the extraction by 662%, 225% and 150% times over the unaided, only cellulase 'Onozuka R-10' treated and only sonication treated samples respectively. The sonication parameters (duration, cycle and amplitude) during the coupled operation were optimized using response surface methodology (RSM). Derivative UV-visible spectra (i.e., dA/dλ and d(2)A/dλ(2) against λ), FTIR analysis, and DPPH scavenging test suggested that the reported extraction protocol did not affect the molecular structure and bioactivity of the extracted lycopene. The influence of sonication on the probable structural modulation (through UV-visible spectral analysis) and activity of the enzyme were also analyzed. A plausible mechanism is proposed for the enhanced extraction achieved via the coupled system.
番茄红素被赋予了多种生物保护作用，主要归因于其独特的分子结构。本文报道了同时利用两种绿色化学工具，即超声和生物催化，从番茄皮中实验室规模提取番茄红素。与未处理、仅用纤维素酶“Onozuka R-10”处理和仅超声处理的样品相比，耦合体系使提取率分别提高了 662%、225%和 150%。在耦合操作过程中，使用响应面法（RSM）优化了超声参数（持续时间、周期和幅度）。导数紫外-可见光谱（即 dA/dλ 和 d(2)A/dλ(2) 与 λ 的关系）、FTIR 分析和 DPPH 清除试验表明，所报道的提取方案没有影响提取的番茄红素的分子结构和生物活性。还分析了超声对可能的结构调制（通过紫外-可见光谱分析）和酶活性的影响。提出了一种通过耦合系统实现增强提取的可能机制。
