Demartini Diogo Ribeiro, Carlini Célia Regina, Thelen Jay J
Department of Biophysics, Center of Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Methods Mol Biol. 2011;775:93-115. doi: 10.1007/978-1-61779-237-3_6.
Proteomics aimed at addressing sub cellular fractions, such as chloroplasts, are a complex challenge. In the past few years, several studies in different laboratories have identified and, more recently, quantified, thousands of proteins within whole chloroplasts or chloroplast fractions. A considerable number of these studies are available for querying, using online resources, such as databases containing the proteins identified, encoding genes, acquired spectra, and phosphopeptides. The main purpose of this review is to identity and highlight useful features of these online resourses, mainly focused in proteomics databases related to chloroplast research in Arabidopsis thaliana. Several web sites were consulted. Among them, 11 were selected and discussed herein. The databases were classified into Plastid Databases, General Organelle Proteome Databases, and General Arabidopsis Proteome Databases. Special care was taken to present information regarding protein identification, protein quantification, and data integration. A selected list of online resources is presented in two tables. The databases analyzed are a useful source of information for researchers in the plastid organelle and plant proteomics fields.
针对亚细胞组分（如叶绿体）的蛋白质组学是一项复杂的挑战。在过去几年中，不同实验室的多项研究已经鉴定出，并且最近还对整个叶绿体或叶绿体组分中的数千种蛋白质进行了定量分析。利用在线资源（如包含已鉴定蛋白质、编码基因、获得的光谱和磷酸化肽段的数据库），可以查询到相当数量的此类研究。本综述的主要目的是识别并突出这些在线资源的有用特征，主要聚焦于与拟南芥叶绿体研究相关的蛋白质组学数据库。查阅了多个网站。其中，挑选了11个在此进行讨论。这些数据库被分为质体数据库、通用细胞器蛋白质组数据库和通用拟南芥蛋白质组数据库。在呈现蛋白质鉴定、蛋白质定量和数据整合的信息时格外谨慎。两张表格中列出了一份精选的在线资源列表。所分析的数据库是质体细胞器和植物蛋白质组学领域研究人员的有用信息来源。
