Tanz Sandra K, Small Ian
Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, WA, Australia.
Methods Mol Biol. 2011;774:243-80. doi: 10.1007/978-1-61779-234-2_16.
Numerous experimental and in silico approaches have been developed for attempting to identify the -subcellular localisation of proteins. Approximately 2,000-4,000 proteins are thought to be targeted to plastids in plants, but a complete and unambiguous catalogue has yet to be drawn up. This article reviews the various prediction methods that identify plastid targeting sequences, and those that can help estimate location and topology within the plastid or plastid membranes. The most successful approaches are described in detail, with detailed notes to help avoid common pitfalls and advice on interpreting conflicting or ambiguous results. In most cases, it is best to try multiple approaches, and we also cover the powerful new integrated databases that provide a selected blend of experimental data and predictions.
为了尝试确定蛋白质的亚细胞定位，已经开发了许多实验方法和计算机模拟方法。据认为，植物中约有2000 - 4000种蛋白质靶向质体，但尚未编制出完整且明确的目录。本文综述了识别质体靶向序列的各种预测方法，以及有助于估计质体或质体膜内位置和拓扑结构的方法。详细描述了最成功的方法，并给出了详细注释，以帮助避免常见错误，并就解释相互矛盾或不明确的结果提供建议。在大多数情况下，最好尝试多种方法，我们还介绍了强大的新综合数据库，这些数据库提供了实验数据和预测的精选组合。
