酵母中组蛋白H3置换动力学的全基因组测量
Genome-wide measurement of histone H3 replacement dynamics in yeast.
作者信息
Rando Oliver J
机构信息
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA.
出版信息
Methods Mol Biol. 2011;759:41-60. doi: 10.1007/978-1-61779-173-4_3.
Abstract
Chromatin plays critical roles in processes governed in different timescales - responses to environmental changes require rapid plasticity, while long-term stability through multiple cell generations requires epigenetically heritable chromatin. Understanding the dynamic behavior of chromatin is of great interest for fields ranging from transcriptional regulation through meiosis and gametogenesis. Here, we describe a protocol for measuring histone replacement rates genome wide in the budding yeast Saccharomyces cerevisiae. With suitable modifications, this protocol could be applied to other organisms, or to replacement dynamics of other DNA-associated proteins.
摘要
染色质在不同时间尺度调控的过程中发挥着关键作用——对环境变化的响应需要快速的可塑性，而多代细胞的长期稳定性则需要表观遗传可遗传的染色质。从转录调控到减数分裂和配子发生等领域，了解染色质的动态行为都具有极大的研究价值。在此，我们描述了一种在芽殖酵母酿酒酵母中全基因组测量组蛋白替换率的方法。经过适当修改，该方法可应用于其他生物，或其他与DNA相关蛋白质的替换动态研究。
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