Zuccari D A P C, Castro R, Gelaleti G B, Mancini U M
Faculdade de Medicina de São José do Rio Preto, SP, Brasil.
Genet Mol Res. 2011;10(3):1522-32. doi: 10.4238/vol10-3gmr1145.
The use of prognostic markers for mammary cancer is important for routine diagnosis and research. Interleukin-8 (IL-8) is a chemotactic cytokine, produced by several cell types in response to inflammation. The expression, regulation and function of IL-8 in dogs are little known. Recent studies have associated angiogenesis and inflammatory processes with tumor malignancy. We investigated a possible correlation between IL-8 expression and mammary tumor prognosis in female dogs. IL-8 expression was measured in 50 dogs with mammary neoplasia by immunohistochemistry and real-time PCR. Immunohistochemical staining was done with anti-IL-8 antibodies and PCR amplifications were performed in a 7500 Fast Real-Time PCR system. Gene expression stability was analyzed by the geNorm software. Quantitative real-time PCR showed that IL-8 expression decreased in malignant mammary cells compared to normal mammary tissue, while weak immunostaining was associated with a diagnosis of carcinoma. Complementing earlier studies on IL-8 expression in several types of cancer, including mammary cancer, we conclude that IL-8 has potential for use as a prognostic marker for canine mammary neoplasia.
乳腺癌预后标志物的应用对于常规诊断和研究至关重要。白细胞介素-8（IL-8）是一种趋化细胞因子，由多种细胞类型在炎症反应中产生。犬类中IL-8的表达、调控和功能鲜为人知。最近的研究将血管生成和炎症过程与肿瘤恶性程度联系起来。我们研究了雌性犬中IL-8表达与乳腺肿瘤预后之间的可能相关性。通过免疫组织化学和实时PCR检测了50只患有乳腺肿瘤的犬的IL-8表达。用抗IL-8抗体进行免疫组织化学染色，并在7500快速实时PCR系统中进行PCR扩增。通过geNorm软件分析基因表达稳定性。定量实时PCR显示，与正常乳腺组织相比，恶性乳腺细胞中IL-8表达降低，而弱阳性免疫染色与癌的诊断相关。作为对包括乳腺癌在内的几种癌症中IL-8表达早期研究的补充，我们得出结论，IL-8有潜力用作犬乳腺肿瘤的预后标志物。
