Zuccari D A P C, Castro R, Gavioli A F, Mancini U M, Frade C S, Leonel C
Laboratório de Investigação Molecular do Câncer, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brasil.
Genet Mol Res. 2012 Jan 27;11(1):153-65. doi: 10.4238/2012.January.27.2.
Caveolin-1 (Cav-1) is a structural protein present in invaginations of the cell membrane. In human breast cancer, the cav-1 gene is believed to be a tumor suppressor gene associated with inhibition of tumor metastasis. However, little is known about its expression, regulation and function in canine mammary tumors. Expression levels of cav-1 were investigated using real-time PCR and immunohistochemical detection with an anti-human Cav-1 antibody. Gene expression stability of different samples was analyzed using the geNorm software. Mammary tumors from 51 female dogs were compared to normal mammary tissue from 10 female dogs. Malignant mammary cells showed a loss of Cav-1 expression by quantitative RT-PCR and weak Cav-1 staining by immunohistochemistry compared to normal mammary gland tissue. There was a significant relationship between outcome and immunostaining as well as with tumor size, indicating that caveolin subexpression has a positive predictive value and is related to higher survival and smaller tumor size. Our findings indicate that Cav-1 is a potential prognostic marker for canine mammary tumors.
小窝蛋白-1（Cav-1）是一种存在于细胞膜内陷处的结构蛋白。在人类乳腺癌中，cav-1基因被认为是一种与抑制肿瘤转移相关的肿瘤抑制基因。然而，关于其在犬乳腺肿瘤中的表达、调控及功能却知之甚少。使用实时PCR和抗人Cav-1抗体的免疫组织化学检测方法研究了cav-1的表达水平。使用geNorm软件分析了不同样本的基因表达稳定性。将51只雌性犬的乳腺肿瘤与10只雌性犬的正常乳腺组织进行了比较。与正常乳腺组织相比，恶性乳腺细胞通过定量RT-PCR显示Cav-1表达缺失，通过免疫组织化学显示Cav-1染色较弱。结果与免疫染色以及肿瘤大小之间存在显著关系，表明小窝蛋白低表达具有阳性预测价值，并且与较高的生存率和较小的肿瘤大小相关。我们的研究结果表明，Cav-1是犬乳腺肿瘤的一种潜在预后标志物。
