Vianna G R, Cunha N B, Murad A M, Rech E L
Laboratório de Transferência e Expressão de Genes, Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília, DF, Brasil.
Genet Mol Res. 2011;10(3):1733-52. doi: 10.4238/vol10-3gmr1476.
Plants present various advantages for the production of biomolecules, including low risk of contamination with prions, viruses and other pathogens, scalability, low production costs, and available agronomical systems. Plants are also versatile vehicles for the production of recombinant molecules because they allow protein expression in various organs, such as tubers and seeds, which naturally accumulate large amounts of protein. Among crop plants, soybean is an excellent protein producer. Soybean plants are also a good source of abundant and cheap biomass and can be cultivated under controlled greenhouse conditions. Under containment, the plant cycle can be manipulated and the final seed yield can be maximized for large-scale protein production within a small and controlled area. Exploitation of specific regulatory sequences capable of directing and accumulating recombinant proteins in protein storage vacuoles in soybean seeds, associated with recently developed biological research tools and purification systems, has great potential to accelerate preliminary characterization of plant-derived biopharmaceuticals and industrial macromolecules. This is an important step in the development of genetically engineered products that are inexpensive and safe for medicinal, food and other uses.
植物在生物分子生产方面具有多种优势，包括感染朊病毒、病毒和其他病原体的风险低、可扩展性、生产成本低以及有可用的农艺系统。植物也是生产重组分子的通用载体，因为它们允许在各种器官（如块茎和种子）中表达蛋白质，这些器官天然会积累大量蛋白质。在农作物中，大豆是出色的蛋白质生产者。大豆植株也是丰富且廉价生物质的良好来源，并且可以在可控的温室条件下种植。在隔离条件下，植物生长周期可以得到控制，最终种子产量可以最大化，以便在小面积且可控的区域内进行大规模蛋白质生产。利用能够在大豆种子的蛋白质储存液泡中指导和积累重组蛋白的特定调控序列，结合最近开发的生物学研究工具和纯化系统，具有极大潜力加速对植物源生物制药和工业大分子的初步表征。这是开发对医药、食品和其他用途而言既廉价又安全的基因工程产品的重要一步。
