多功能壳聚糖纳米凝胶用于药物递送、生物成像和生物传感的多功能化。
Multifunctional chitin nanogels for simultaneous drug delivery, bioimaging, and biosensing.
机构信息
Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, India.
出版信息
ACS Appl Mater Interfaces. 2011 Sep;3(9):3654-65. doi: 10.1021/am200844m. Epub 2011 Sep 12.
In this work, we developed biodegradable chitin nanogels (CNGs) by controlled regeneration method. For multifunctionalization, we have conjugated CNGs with MPA-capped-CdTe-QDs (QD-CNGs) for the in vitro cellular localization studies. In addition, the Bovine Serum Albumin (BSA) was loaded on to QD-CNGs (BSA-QD-CNGs). The CNGs, QD-CNGs, and BSA-QD-CNGs were well-characterized by SEM and AFM, which shows that the nanogels are in the range of <100 nm. These were further characterized by FT-IR and Cyclic Voltametry. The cytocompatibility assay showed that the nanogels are nontoxic to L929, NIH-3T3, KB, MCF-7, PC3, and VERO cells. The cell uptake studies of the QD-CNGs were analyzed, which showed retention of these nanogels inside the cells (L929, PC3, and VERO). In addition, the protein loading efficiency of the nano gels has also been analyzed. Our preliminary studies reveal that these multifunctionalized nanogels could be useful for drug delivery with simultaneous imaging and biosensing.
在这项工作中，我们通过控制再生的方法开发了可生物降解的壳聚糖纳米凝胶（CNGs）。为了实现多功能化，我们将壳聚糖纳米凝胶与 MPA 封端的 CdTe-QDs（QD-CNGs）偶联，用于体外细胞定位研究。此外，牛血清白蛋白（BSA）被负载到 QD-CNGs（BSA-QD-CNGs）上。通过 SEM 和 AFM 对 CNGs、QD-CNGs 和 BSA-QD-CNGs 进行了很好的表征，结果表明纳米凝胶的尺寸<100nm。通过傅里叶变换红外光谱（FT-IR）和循环伏安法（Cyclic Voltametry）对其进行了进一步的表征。细胞相容性试验表明，纳米凝胶对 L929、NIH-3T3、KB、MCF-7、PC3 和 VERO 细胞均无毒性。对 QD-CNGs 的细胞摄取研究进行了分析，结果表明这些纳米凝胶在细胞内被保留（L929、PC3 和 VERO）。此外，还分析了纳米凝胶的蛋白质负载效率。我们的初步研究表明，这些多功能纳米凝胶可用于药物输送，同时进行成像和生物传感。
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