从中国黑龙江省分离的 3 株旋毛虫分离株的分子鉴定
Molecular identification of three Trichinella isolates from Heilongjiang Province, People's Republic of China.
机构信息
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, PR China.
出版信息
Exp Parasitol. 2011 Nov;129(3):299-302. doi: 10.1016/j.exppara.2011.08.009. Epub 2011 Aug 16.
DNAs of Trichinella dog isolate (HC), Trichinella swine isolate (HH) and Trichinella cat isolate (SW), obtained from Heilongjiang Province, were amplified by the fragments of 18S rDNA and ITS2. Two reference strains, Trichinella spiralis (ISS3) and Trichinella nativa (ISS10) were used for sequence comparison. Sequence and dendrogram analysis indicated that HC belonged to T. nativa and HH together with SW belonged to T. spiralis. This method permits rapid species identification of Trichinella isolates, although further evaluation is required before precisely identification.
从黑龙江省获得的旋毛虫犬株（HC）、旋毛虫猪株（HH）和旋毛虫猫株（SW）的 DNA 通过 18S rDNA 和 ITS2 片段进行扩增。使用了两个参考株，旋毛虫（ISS3）和天然旋毛虫（ISS10）用于序列比较。序列和系统发育分析表明，HC 属于天然旋毛虫，而 HH 和 SW 一起属于旋毛虫。尽管在进行精确鉴定之前还需要进一步评估，但该方法允许快速鉴定旋毛虫分离株的种类。
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