通过对5S核糖体DNA基因间隔区进行扩增和测序来对旋毛虫分离株进行简单的种属鉴定。
Simple species identification of Trichinella isolates by amplification and sequencing of the 5S ribosomal DNA intergenic spacer region.
作者信息
De Bruyne Aymeric, Yera Hélène, Le Guerhier Franck, Boireau Pascal, Dupouy-Camet Jean
机构信息
Trichinella National Reference Center, Assistance Publique-Hôpitaux de Paris, Hôpital Cochin, R. Descartes University, 27 rue du Fbrg St. Jacques, 75014 Paris, France.
出版信息
Vet Parasitol. 2005 Sep 5;132(1-2):57-61. doi: 10.1016/j.vetpar.2005.05.026.
We developed a PCR-based assay using a single primer pair to amplify the 5S ribosomal DNA intergenic spacer region to identify Trichinella isolates. In our method, amplified products are directly sequenced on both strands and compared to GenBank sequences. Using this method, we were able to identify Trichinella spiralis, T. britovi and T. nativa. This method permits rapid species identification of Trichinella isolates; however, further evaluation is required before recommending this approach for routine use.
我们开发了一种基于聚合酶链反应（PCR）的检测方法，使用一对引物扩增5S核糖体DNA基因间隔区，以鉴定旋毛虫分离株。在我们的方法中，扩增产物直接进行双链测序，并与GenBank序列进行比较。使用这种方法，我们能够鉴定出旋毛虫、布氏旋毛虫和本地旋毛虫。该方法能够快速鉴定旋毛虫分离株；然而，在推荐将此方法用于常规检测之前，还需要进一步评估。
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