Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Rumburská 89, 27 721 Liběchov, Czech Republic.
Mol Phylogenet Evol. 2011 Dec;61(3):854-65. doi: 10.1016/j.ympev.2011.08.003. Epub 2011 Aug 16.
The genus Pangio is one of the most species-rich of the loach family Cobitidae and widespread across South and Southeast Asia. Its species diversity has never been studied under a clear phylogenetic approach, but four 'species-groups' were proposed according to the most obvious morphological characters. We present here phylogenetic analyses of the genus Pangio based on sequence data of the mitochondrial cytochrome b gene, the nuclear recombination-activating gene 1 (RAG 1) and a combined dataset of 109 specimens from 18 morphologically identified species across the whole distribution area of the genus. Our data reveal the existence of three major lineages within Pangio. Two of our major lineages were congruent with formerly proposed species-groups, the remaining two species-groups together formed the third major lineage; herein we refer to the lineages as to anguillaris-group, kuhlii-oblonga group and shelfordii-group. The application of a molecular clock dated the age of the three lineages to 33-29 million years. At the species level, our data suggest about 30 distinct lineages, indicating that there is a high number of undescribed species within Pangio. The use of Pangio to address biogeographic questions is demonstrated with the example of the shelfordii-group, which is distributed across Sundaland.
攀鲈属是鳅科中物种最丰富的属之一，广泛分布于南亚和东南亚。其物种多样性从未通过明确的系统发育方法进行研究，但根据最明显的形态特征提出了四个“物种群”。我们根据来自攀鲈属整个分布区的 109 个形态鉴定标本的线粒体细胞色素 b 基因、核重组激活基因 1 (RAG 1) 和综合数据集，在此提出了攀鲈属的系统发育分析。我们的数据揭示了攀鲈属内存在三个主要谱系。我们的两个主要谱系与以前提出的物种群一致，其余两个物种群共同形成了第三个主要谱系；在这里，我们将这些谱系称为鳗形群、长丝鲈群和史氏攀鲈群。分子钟的应用将这三个谱系的年龄定在 3300 万至 2900 万年前。在种的水平上，我们的数据表明有大约 30 个不同的谱系，这表明攀鲈属内有大量未被描述的物种。攀鲈属被用来解决生物地理问题的一个例子是史氏攀鲈群，它分布在巽他陆架。
