Azizov Iu M, Semavin I E, Kazakov I V, Nuzhdin I L, Makarshev I M
Ter Arkh. 1990;62(2):99-104.
In patients with diffuse purulent peritonitis, the total pool of an acid-soluble blood plasma fraction (ABPF) considerably increases as compared to that in normals (donors). The present paper is concerned with the role of proteolytic activity and lysis of bacterial cells and cells of different human tissues in the formation of the ABPF in patients with peritonitis. For this purpose the normals' blood and its separate components were treated with various enzymes (trypsin, pronase, chemotrypsin, papain, elastase, alpha-amylase) to measure the ABPF. The pattern of changes detectable on such a treatment was compared with the tendency of changes seen in patients with peritonitis. Measurements were also made of acid-soluble fractions in suspensions of bacterial cells and cells of the pancreas, liver and heart muscle tissue of man. It has been found that on proteolysis of plasma proteins and membrane proteins of blood cells there form products contained by the ABPF. Such products formed in the greatest amount as a result of treatment with pronase. Products of the lysis of bacterial cells may be also contained by the ABPF of peritonitis patients. The possibility of detecting proteolytic degradation and cellular disintegration according to the spectral characteristics of separate fractions of the ABPF is discussed.
在弥漫性化脓性腹膜炎患者中，与正常人（献血者）相比，酸溶性血浆组分（ABPF）的总量显著增加。本文关注蛋白水解活性以及细菌细胞和人体不同组织细胞的裂解在腹膜炎患者ABPF形成中的作用。为此，用各种酶（胰蛋白酶、链霉蛋白酶、胰凝乳蛋白酶、木瓜蛋白酶、弹性蛋白酶、α淀粉酶）处理正常人的血液及其分离组分以测量ABPF。将这种处理后可检测到的变化模式与腹膜炎患者所见的变化趋势进行比较。还对人胰腺、肝脏和心肌组织的细菌细胞和细胞悬液中的酸溶性组分进行了测量。已发现，血浆蛋白和血细胞的膜蛋白发生蛋白水解时会形成ABPF所含的产物。用链霉蛋白酶处理产生的此类产物最多。细菌细胞裂解产物也可能存在于腹膜炎患者的ABPF中。讨论了根据ABPF各分离组分的光谱特征检测蛋白水解降解和细胞解体的可能性。
