Olsson O, Nilsson O, Koncz C
Department of Plant Physiology, University of Umeå, Sweden.
J Biolumin Chemilumin. 1990 Apr-Jun;5(2):79-87. doi: 10.1002/bio.1170050203.
Taking advantage of a specially constructed vector, luciferase LuxA and LuxB subunits were connected in frame to different amino acid linkers to reproduce a series of monomeric luciferase enzymes. A comparison of their activities in E. coli cells demonstrated that the length of the linkers positively affected activity. One luciferase fusion gene was expressed in plant cells, and we showed that this gene activity could be monitored directly without destructive sampling.
利用一种特殊构建的载体，将荧光素酶LuxA和LuxB亚基与不同的氨基酸接头进行读框连接，以重现一系列单体荧光素酶。它们在大肠杆菌细胞中的活性比较表明，接头的长度对活性有正向影响。一个荧光素酶融合基因在植物细胞中表达，并且我们表明该基因活性可以在不进行破坏性取样的情况下直接监测。
