Kutsyĭ M P
Radiats Biol Radioecol. 2011 May-Jun;51(3):328-36.
It has been found that proteases specifically splitting histones are associated with histones from spleen and brain nuclei of 4- and 26-month-old rats. The activity ofproteases isolated together with histones increases after irradiation of rats with 10 Gy The activation degree of these proteases depends on the animal age and postradiation period. Activation ofhistone-associated proteases by means of gamma-radiation is more pronounced in spleen nuclei from old rats than from the young ones. Irradiation of animals has been found to reduce histone H1 and core histone contents in the spleen and brain nuclei of both young and old rats. The radiation-induced proteolysis ofhistone H1 and core histones in spleen and brain nuclei leads to chromatin deconden-sation and DNA degradation by nucleases. The activity of histone-associated proteases is substantially higher in the nuclei of intensively proliferating spleen cells than in the brain nuclei. The experimental data indicate that histone-associated proteases participate in the regulation of DNA transcription, replication, and degradation.
已发现，特异性裂解组蛋白的蛋白酶与4月龄和26月龄大鼠脾脏及脑细胞核中的组蛋白相关。与组蛋白一起分离得到的蛋白酶活性在大鼠接受10 Gy照射后增加。这些蛋白酶的激活程度取决于动物年龄和辐射后时期。通过γ射线辐射激活组蛋白相关蛋白酶在老年大鼠脾脏细胞核中比在幼年大鼠中更明显。已发现动物照射会降低幼年和老年大鼠脾脏及脑细胞核中组蛋白H1和核心组蛋白的含量。脾脏和脑细胞核中辐射诱导的组蛋白H1和核心组蛋白的蛋白水解导致染色质解聚和核酸酶对DNA的降解。在增殖活跃的脾脏细胞的细胞核中，组蛋白相关蛋白酶的活性明显高于脑细胞核。实验数据表明，组蛋白相关蛋白酶参与DNA转录、复制和降解的调控。
