The W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA.
Vector Borne Zoonotic Dis. 2011 Nov;11(11):1493-7. doi: 10.1089/vbz.2011.0662. Epub 2011 Aug 25.
In some mosquito species the conditions experienced by larvae during development have been shown to lead to changes in susceptibility to various arboviruses in the adult female. Since laboratory mosquitoes are generally reared under ideal conditions, mosquito vector competence experiments in the laboratory may not accurately reflect vector?virus relationships in nature. We examined the consequences of larval nutritional stress on Culex tarsalis vector competence for West Nile virus (WNV). Larval nutrition deprivation resulted in increased development time, decreased pupation and emergence rates, and smaller adult female body size. However, infection, dissemination, and transmission rates for WNV at 5, 7, and 14 days postfeeding were not consistently affected. These results suggest that larval nutritional rearing protocols are not a major factor in laboratory estimates of WNV vector competence in Cx. tarsalis.
在一些蚊子物种中，幼虫在发育过程中所经历的条件已被证明会导致成年雌性蚊子对各种虫媒病毒的易感性发生变化。由于实验室蚊子通常在理想的条件下饲养，因此实验室中的蚊子媒介能力实验可能无法准确反映自然界中的媒介-病毒关系。我们研究了幼虫营养胁迫对库蚊传播西尼罗河病毒（WNV）能力的影响。幼虫营养剥夺导致发育时间延长、化蛹和羽化率降低以及成年雌性体型变小。然而，WNV 的感染、传播和传递率在 5、7 和 14 天的喂养后并没有受到一致的影响。这些结果表明，在实验室估计库蚊传播西尼罗河病毒的能力时，幼虫营养饲养方案并不是一个主要因素。
