Department of Biology, University of Lund, Sölvegatan, LU, Sweden.
Prog Brain Res. 2011;194:253-62. doi: 10.1016/B978-0-444-53815-4.00002-9.
Here we describe our attempts to study the interaction of nanomodified surfaces with neurons and macrophages. Surfaces with nano-sized topographies produced by UV lithography, electrochemical etching, nanoimprint lithography, microdispensing, or by electrospinning of plastic nanofibers or by making plastic replicas of the extracellular matrix with nanoresolution were found to guide neurite outgrowth extending from the dorsal root and the superior cervical ganglion in tissue culture. Ordered arrays of nanowires acted as particularly potent guides for the neurites. Loose nanowires activated the macrophages. We conclude that relatively simple nanomodifications of surfaces can be utilized to guide neurites. This property could potentially be applied to guide neurite outgrowth on implants in the nervous system intended for recordings of electrical and/or chemical activities.
在这里，我们描述了我们研究纳米修饰表面与神经元和巨噬细胞相互作用的尝试。通过紫外光刻、电化学蚀刻、纳米压印光刻、微分配或静电纺丝塑料纳米纤维或用纳米分辨率制作细胞外基质的塑料复制品制造的具有纳米级形貌的表面被发现可以引导组织培养中的背根和颈上交感神经节中的神经突生长。纳米线的有序阵列特别有效地引导了神经突。松散的纳米线激活了巨噬细胞。我们得出结论，相对简单的表面纳米修饰可用于引导神经突。这一特性可能被应用于引导神经系统植入物中的神经突生长，以便记录电和/或化学活性。
