State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, PR China.
J Genet Genomics. 2011 Aug 20;38(8):357-71. doi: 10.1016/j.jgg.2011.07.004. Epub 2011 Jul 23.
Puccinia striiformis f. sp. tritici (Pst) causes stripe rust, one of the most important diseases of wheat worldwide. cDNA libraries had been constructed from urediniospores, germinated urediniospores and haustoria. However, little is known about the expression patterns of the genes related to the infection process and sporulation of the pathogen. In this study, a custom oligonucleotide microarray was constructed using sequences of 442 gene transcripts selected from Pst cDNA libraries. The expression patterns of the genes were determined by hybridizing the microarray with cDNA from Pst in vitro and Pst-infected wheat leaves. The time course study identified 55 transcripts that were differentially expressed during the infection process in a compatible interaction. They were identified to have functions related to the following biological processes, including carbohydrate and lipid metabolism, energy, cell signaling, protein synthesis, cell structure and division. In an incompatible interaction, 17 transcripts of the pathogen were differentially expressed in resistant wheat leaves inoculated with an avirulent Pst race, ten of which had similar expression patterns to those in the compatible interaction. Several candidates for pathogenicity and virulence/avirulence related genes were also identified. The results of quantitative real-time PCR validated the expression patterns of some selected genes. The study demonstrates that the custom oligonucleotide microarray technology is useful to determine the expression patterns of the pathogen genes involved in different types of the host-pathogen interactions and stages of development.
小麦条锈菌（Puccinia striiformis f. sp. tritici，Pst）引起条锈病，是全球小麦最重要的病害之一。已从夏孢子、萌发夏孢子和吸器中构建了 cDNA 文库。然而，对于与病原菌侵染过程和孢子形成相关的基因的表达模式知之甚少。本研究使用从 Pst cDNA 文库中选择的 442 个基因转录本的序列构建了定制的寡核苷酸微阵列。通过将微阵列与体外 Pst cDNA 和 Pst 感染的小麦叶片杂交，确定了基因的表达模式。时间过程研究鉴定了在亲和互作中侵染过程中差异表达的 55 个转录本。它们被鉴定为具有与以下生物过程相关的功能，包括碳水化合物和脂质代谢、能量、细胞信号转导、蛋白质合成、细胞结构和分裂。在非亲和互作中，接种无毒 Pst 小种的抗病小麦叶片中差异表达了 17 个病原菌转录本，其中 10 个的表达模式与亲和互作中相似。还鉴定了一些与致病性和毒性/无毒相关的基因候选。定量实时 PCR 的结果验证了一些选定基因的表达模式。该研究表明，定制的寡核苷酸微阵列技术可用于确定参与不同类型的寄主-病原互作和发育阶段的病原菌基因的表达模式。
