Unité de pathologie cellulaire et génétique UPRES-EA2493, UFR Paris Ile de France Ouest PRES Universud Paris, Université de Versailles-Saint Quentin en Yvelines, Versailles, France.
Placenta. 2011 Oct;32(10):771-7. doi: 10.1016/j.placenta.2011.06.029. Epub 2011 Aug 24.
During pregnancy, placental growth allows the adaptation of the feto-maternal unit to fetal requirements. Placental alkaline phosphatase (PLAP) is a phosphomonoesterase produced increasingly until term by the placenta and also ectopically in some tumors. To precise the role of this enzyme in the placenta, we analyzed the genome wide expression profile of HTR-8/Svneo trophoblastic cells after overexpression of the alkaline phosphatase gene (ALPP). We showed that ALPP overexpression mainly altered expression of genes implicated in cellular growth and proliferation. These results were confirmed by the study of cellular effects in HTR-8/Svneo cells overexpressing ALPP and in HTR-8/Svneo cells in which ALPP expression was suppressed by siRNA. We showed that PLAP exerts a positive effect on DNA replication and acts as a proliferative factor in trophoblastic cells.
在妊娠期间，胎盘的生长允许胎儿-母体单位适应胎儿的需求。胎盘碱性磷酸酶（PLAP）是一种磷酸单酯酶，由胎盘不断产生，直到足月，也在一些肿瘤中异位产生。为了明确这种酶在胎盘中的作用，我们分析了碱性磷酸酶基因（ALPP）过表达后 HTR-8/Svneo 滋养层细胞的全基因组表达谱。我们发现，ALPP 过表达主要改变了与细胞生长和增殖相关的基因表达。这些结果通过在 HTR-8/Svneo 细胞中过表达 ALPP 以及通过 siRNA 抑制 ALPP 表达的 HTR-8/Svneo 细胞中研究细胞效应得到了证实。我们表明，PLAP 对 DNA 复制有积极影响，并在滋养层细胞中作为增殖因子发挥作用。
