Nozawa S, Narisawa S, Iizuka R, Fukasawa T, Kohji T, Nakane P K, Hirano K, Millan J L
Department of Obstetrics and Gynecology, Keio University School of Medicine, Japan.
Cell Biochem Funct. 1989 Jul;7(3):227-32. doi: 10.1002/cbf.290070312.
The mechanism of placental alkaline phosphatase (PLAP) induction by prednisolone in a uterine cervical epidermoid cancer cell line SKG-IIIa was investigated in vitro by enzyme-cytochemistry, enzyme immunoassay, Northern and Southern blot analysis, and in situ hybridization. Enzyme-cytochemical alkaline phosphatase (ALP) staining and immunoassay revealed increased levels of PLAP (heat-stable ALP) in prednisolone-treated cells. Northern blot analysis and in situ hybridization showed increased amounts of PLAP mRNA. Southern blot analysis indicated that PLAP was not a product of an amplified or rearranged gene. These findings suggest that the induction of PLAP mRNA in SKG-IIIa cells by prednisolone in turn increased the levels of PLAP.
通过酶细胞化学、酶免疫测定、Northern和Southern印迹分析以及原位杂交等方法，在体外研究了泼尼松龙诱导子宫颈表皮样癌细胞系SKG-IIIa中胎盘碱性磷酸酶（PLAP）的机制。酶细胞化学碱性磷酸酶（ALP）染色和免疫测定显示，泼尼松龙处理的细胞中PLAP（热稳定ALP）水平升高。Northern印迹分析和原位杂交显示PLAP mRNA的量增加。Southern印迹分析表明PLAP不是扩增或重排基因的产物。这些发现表明，泼尼松龙诱导SKG-IIIa细胞中PLAP mRNA的表达，进而增加了PLAP的水平。
