Department of Radiology, Shanghai First People's Hospital, Medical College, Shanghai Jiaotong University, Shanghai 200080, China.
Magn Reson Imaging. 2011 Oct;29(8):1101-9. doi: 10.1016/j.mri.2011.07.003. Epub 2011 Aug 24.
The objective was to analyze the correlation of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) with vascular endothelial growth factor (VEGF) protein expression and to assess the potential application of DCE-MRI to the rabbit cerebrospinal fluid (CSF) metastasis model.
Thirty New Zealand rabbits were divided into experimental and control groups. In the experimental group, VX2 tumor cells were injected into the subarachnoid space at the plane of cisterna magna in 24 rabbits. In the control group, physiological saline was injected into the subarachnoid space at the plane of cisterna magna in six rabbits. DCE-MRI was performed at multiple time points, and several pharmacokinetic parameters, including K(trans), K(ep) and V(e), were calculated. Also, VEGF levels in plasma and CSF were evaluated by enzyme-linked immunosorbent assay prior to DCE-MRI examination. After DCE-MRI examination, the rabbits were sacrificed, and the corresponding tumor specimens were harvested. Hematoxylin-eosin staining and VEGF immunohistochemical staining were carried out, and VEGF expression in the specimens was evaluated by the immunohistochemical scoring system.
Vascular endothelial growth factor positive staining was localized in the cytoplasm and cell membranes of tumor cells, as well as in a subset of epithelial cells. Both VEGF immunohistochemical scores and VEGF expression in CSF and plasma exhibited positive correlations with K(trans) and K(ep) values as demonstrated by rank correlation statistical analysis.
Vascular endothelial growth factor expression in plasma and CSF in the CSF metastasis model was higher than in normal tissues. Therefore, DCE-MRI reliably indicated VEGF expression in the rabbit CSF metastasis model.
分析动态对比增强磁共振成像（DCE-MRI）与血管内皮生长因子（VEGF）蛋白表达的相关性，并评估 DCE-MRI 在兔脑脊液（CSF）转移模型中的潜在应用。
30 只新西兰兔分为实验组和对照组。实验组 24 只兔蛛网膜下腔在枕大池平面注射 VX2 肿瘤细胞，对照组 6 只兔蛛网膜下腔在枕大池平面注射生理盐水。在多个时间点进行 DCE-MRI 检查，计算多个药代动力学参数，包括 K(trans)、K(ep)和 V(e)。在 DCE-MRI 检查前，通过酶联免疫吸附试验评估血浆和 CSF 中的 VEGF 水平。DCE-MRI 检查后，处死兔，采集相应的肿瘤标本。进行苏木精-伊红染色和 VEGF 免疫组织化学染色，通过免疫组织化学评分系统评估标本中的 VEGF 表达。
血管内皮生长因子阳性染色定位于肿瘤细胞的细胞质和细胞膜，以及部分上皮细胞。通过秩相关统计分析，VEGF 免疫组织化学评分和 CSF 及血浆中的 VEGF 表达均与 K(trans)和 K(ep)值呈正相关。
CSF 转移模型中血浆和 CSF 中的 VEGF 表达高于正常组织。因此，DCE-MRI 可靠地反映了兔 CSF 转移模型中 VEGF 的表达。
