[山奈酚诱导人食管鳞状癌细胞Eca-109凋亡及其机制]

[Kaempferol-induced apoptosis of human esophageal squamous carcinoma Eca-109 cells and the mechanism].

作者信息

Li Rui-jun, Mei Jia-zhuan, Liu Gui-ju

机构信息

Department of Medical Oncology, Zhengzhou Peoples Hospital, Zhengzhou, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2011 Aug;31(8):1440-2.

PMID:21868342

Abstract

OBJECTIVE

To evaluate the growth-inhibiting and pro-apoptotic effect of kaempferol in human esophageal squamous carcinoma Eca-109 cells and explore the mechanism.

METHODS

The effect of kaempferol on Eca-109 cell proliferation in vitro was measured by MTT assay. TUNEL staining was used to detect the cell apoptosis following kaempferol treatment. The changes in Bax and Bcl-2 mRNA expressions in response to kaempferol treatment were determined by RT-PCR, and the caspase-3 and caspase-9 activities were evaluated using colorimetric assay.

RESULTS

Kaempferol significantly inhibited Eca-109 cell proliferation (P<0.05) in a concentration-dependent manner and induced obvious cell apoptosis. RT-PCR showed that after kaempferol treatment caused up-regulated Bax and down-regulated Bcl-2 mRNA expression. The colorimetric assay revealed significantly increased caspase-3 and caspase-9 activities in Eca-109 cells following kaempferol treatment (P<0.01).

CONCLUSION

Kaempferol can induce apoptosis of Eca-109 cells via a mitochondria-dependent pathway.

摘要

目的

评估山奈酚对人食管鳞状癌细胞Eca - 109的生长抑制和促凋亡作用，并探讨其机制。

方法

采用MTT法检测山奈酚对Eca - 109细胞体外增殖的影响。运用TUNEL染色检测山奈酚处理后的细胞凋亡情况。通过RT - PCR测定山奈酚处理后Bax和Bcl - 2 mRNA表达的变化，采用比色法评估caspase - 3和caspase - 9的活性。

结果

山奈酚以浓度依赖性方式显著抑制Eca - 109细胞增殖（P<0.05），并诱导明显的细胞凋亡。RT - PCR显示，山奈酚处理后Bax mRNA表达上调，Bcl - 2 mRNA表达下调。比色法显示，山奈酚处理后Eca - 109细胞中caspase - 3和caspase - 9的活性显著增加（P<0.01）。