Centro de Vigilancia Sanitaria Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
Vet Rec. 2011 Oct 1;169(14):362. doi: 10.1136/vr.d4869. Epub 2011 Aug 25.
Two hundred and five isolates of Pasteurella multocida from pigs were phenotypically and genetically characterised by determining their biovar, capsular type, virulence-associated genes and pulsed-field gel electrophoresis (PFGE) profiles. All isolates were identified as P multocida subspecies multocida and most were assigned to biovar 3 (58 per cent) and biovar 2 (39.5 per cent). Biovar 1 represented 2.4 per cent of the isolates. According to the capsular type, the great majority of the isolates (79.0 per cent) belonged to capsular type A, 18.5 per cent belonged to capsular type D and 2.4 per cent were of capsular type F. All isolates harboured ompH, psl, oma87, ptfA, nanB, nanH, tonB, hgbA, sodA and sodC genes, while none of them possessed the transferrin-binding protein gene tbpA. The prevalence of toxA, pfhaA and hgbB genes was variable (7.8, 40.5 and 60.5 per cent of the isolates, respectively). After PFGE typing, isolates of biovar 2 and 3 were grouped in two different clusters (A and B) at a level of 45 per cent similarity. In addition, isolates of biovar 2 and 3 exhibited statistically significant differences (P<0.05) in the virulence-associated hgbB and pfhA genes (biovar 3 was hgbB(+) pfhA(-), while biovar 2 was hgbB- pfhA(+)).
从猪中分离出 205 株多杀性巴氏杆菌，通过确定其生物型、荚膜型、毒力相关基因和脉冲场凝胶电泳 (PFGE) 图谱对其进行了表型和遗传特征分析。所有分离株均被鉴定为多杀性巴氏杆菌亚种多杀性巴氏杆菌，大多数被分配到生物型 3（58%）和生物型 2（39.5%）。生物型 1 占分离株的 2.4%。根据荚膜型，绝大多数分离株（79.0%）属于荚膜型 A，18.5%属于荚膜型 D，2.4%属于荚膜型 F。所有分离株均携带 ompH、psl、oma87、ptfA、nanB、nanH、tonB、hgbA、sodA 和 sodC 基因，而无一携带转铁蛋白结合蛋白基因 tbpA。toxA、pfhaA 和 hgbB 基因的流行率各不相同（分别为 7.8%、40.5%和 60.5%的分离株）。PFGE 分型后，生物型 2 和 3 的分离株在 45%相似度水平上分为两个不同的簇（A 和 B）。此外，生物型 2 和 3 的分离株在毒力相关基因 hgbB 和 pfhA 中存在统计学显著差异（P<0.05）（生物型 3 为 hgbB(+) pfhA(-)，而生物型 2 为 hgbB- pfhA(+））。
