Graham S, Secombes C J
Department of Zoology, University of Aberdeen, UK.
Dev Comp Immunol. 1990 Winter;14(1):59-68. doi: 10.1016/0145-305x(90)90008-3.
The ability of different populations of rainbow trout blood leucocytes to produce MAF following stimulation with Con A/PMA was assessed by the amount of NBT reduction in target macrophages. The effect of varying lymphocyte or macrophage number on MAF production in the presence of a constant number of macrophages or lymphocytes respectively, showed that in both cases MAF activity initially increased with increasing cell number and then plateaued. Macrophages alone did not produce MAF whereas some MAF activity was produced by macrophage-depleted lymphocytes, although significantly lower than in the presence of macrophages. Separation of leucocytes into sIg- and sIg+ cells by panning showed that only sIg- lymphocytes could produce MAF and that macrophages were necessary as accessory cells. These results support the contention that fish lymphocytes can be divided into sIg- T cells and sIg+ B cells.
通过靶巨噬细胞中NBT还原量评估了虹鳟鱼不同群体血液白细胞在Con A/PMA刺激后产生巨噬细胞活化因子（MAF）的能力。分别在巨噬细胞或淋巴细胞数量恒定的情况下，改变淋巴细胞或巨噬细胞数量对MAF产生的影响表明，在这两种情况下，MAF活性最初都随细胞数量增加而升高，然后趋于平稳。单独的巨噬细胞不产生MAF，而去除巨噬细胞的淋巴细胞可产生一些MAF活性，尽管明显低于有巨噬细胞存在时。通过淘选将白细胞分为表面免疫球蛋白阴性（sIg-）和表面免疫球蛋白阳性（sIg+）细胞，结果显示只有sIg-淋巴细胞能产生MAF，且巨噬细胞作为辅助细胞是必需的。这些结果支持了鱼类淋巴细胞可分为sIg-T细胞和sIg+B细胞这一论点。
