Dr. B. R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi 110007, India.
Evid Based Complement Alternat Med. 2011;2011:427031. doi: 10.1093/ecam/nep223. Epub 2011 May 2.
In Indian traditional medicine, Boerhaavia diffusa (punarnava) roots have been widely used for the treatment of dyspepsia, jaundice, enlargement of spleen, abdominal pain and as an anti-stress agent. Pharmacological evaluation of the crude ethanolic extract of B. diffusa roots has been shown to possess antiproliferative and immunomodulatory properties. The extract of B. diffusa was studied for anti-proliferative effects on the growth of HeLa cells and for its effect on cell cycle. Bio-assays of extracts from B. diffusa root showed that a methanol : chloroform fraction (BDF 5) had an antiproliferative effect on HeLa cells. After 48 h of exposure, this fraction at a concentration of 200 μg mL(-1) significantly reduced cell proliferation with visible morphological changes in HeLa cells. Cell cycle analysis suggests that antiproliferative effect of BDF 5 could be due to inhibition of DNA synthesis in S-phase of cell cycle in HeLa cells, whereas no significant change in cell cycle was detected in control cells. The fraction BDF 5 caused cell death via apoptosis as evident from DNA fragmentation and caspase-9 activation. Thus the extract has potential to be evaluated in detail to assess the molecular mechanism-mediated anticancer activities of this plant.
在印度传统医学中，白花丹（punarnava）根被广泛用于治疗消化不良、黄疸、脾肿大、腹痛和作为抗应激剂。对白花丹根的粗乙醇提取物的药理学评价表明，它具有抗增殖和免疫调节特性。研究了白花丹提取物对 HeLa 细胞生长的抗增殖作用及其对细胞周期的影响。从白花丹根提取的生物测定表明，甲醇：氯仿部分（BDF5）对 HeLa 细胞具有抗增殖作用。暴露 48 小时后，该部分在 200μg/mL（-1）浓度下显著降低 HeLa 细胞的增殖，细胞形态发生明显变化。细胞周期分析表明，BDF5 的抗增殖作用可能是由于抑制了 HeLa 细胞 S 期的 DNA 合成，而对照细胞中未检测到细胞周期的显著变化。该部分通过 DNA 片段化和 caspase-9 激活导致细胞凋亡而引起细胞死亡。因此，该提取物具有详细评估的潜力，以评估该植物介导的抗癌活性的分子机制。
