通过抑制L.的分泌型磷脂酶A和[此处原文似乎缺失部分内容]来中和炎症
Neutralization of Inflammation by Inhibiting and Secretory Phospholipase A by Ethanol Extract of L.
作者信息
Giresha Aladahalli S, Pramod Siddanakoppalu N, Sathisha A D, Dharmappa K K
机构信息
Department of Post Graduate Studies and Research in Biochemistry, Post Graduate Centre, Mangalore University, Kodagu, Karnataka, India.
Department of Studies and Research in Biochemistry, Laboratory of Immunomodulation and inflammation Biology, Sahyadri Science College (Autonomous), Kuvempu University, Shimoga, Karnataka, India.
出版信息
Pharmacognosy Res. 2017 Apr-Jun;9(2):174-181. doi: 10.4103/0974-8490.204650.
BACKGROUND
Inflammation is a normal and necessary prerequisite to healing of the injured tissues. Inflammation contributes to all disease process including immunity, vascular pathology, trauma, sepsis, chemical, and metabolic injuries. The secretory phospholipase A (sPLA) is a key enzyme in the production of pro-inflammatory mediators in chronic inflammatory disorders such as rheumatoid arthritis, coronary heart disease, diabetes, and asthma. The sPLA also contribute to neuroinflammatory disorders such as Parkinson's, Alzheimer's, and Crohn's disease.
AIMS
The present study aims to investigate the inhibition of human sPLA by a popular medicinal herb Linn. as a function of anti-inflammatory activity.
MATERIALS AND METHODS
The aqueous and different organic solvents extracts of were prepared and evaluated for human synovial fluid, human pleural fluid, as well as and venom sPLA enzyme inhibition.
RESULTS
Among the extracts, the ethanol extract of (EEBD) showed the highest sPLA inhibition and IC values ranging from 17.8 to 27.5 μg. Further, antioxidant and lipid peroxidation activities of extract were checked using 2,2-diphenyl-1-picrylhydrazyl radical, thiobarbituric acid, and rat liver homogenate. The antioxidant activity of EEBD was more or less directly proportional to sPLA inhibition. Eventually, the extract was subjected to neutralize sPLA-induced mouse paw edema and indirect hemolytic activity. The EEBD showed similar potency in both the cases.
CONCLUSIONS
The findings suggest that the bioactive molecule/s from the EEBD is/are potentially responsible for the observed and sPLA inhibition and antioxidant activity.
SUMMARY
The present study aims to investigate the inhibition of human sPLA by a popular medicinal herb Linn. as a function of anti inflammatory activity. EEBD: Ethanolic extract of boerhaavia diffusa, sPLA: Secretory phospholipase A, HSF: Human synovial fluid, HPF: Human pleural fluid, VRV-PLA2-V: phospholipase A2, NN-PLA-I: phospholipase A.
背景
炎症是受损组织愈合的正常且必要前提。炎症参与所有疾病过程，包括免疫、血管病变、创伤、败血症、化学和代谢损伤。分泌型磷脂酶A（sPLA）是类风湿性关节炎、冠心病、糖尿病和哮喘等慢性炎症性疾病中促炎介质产生的关键酶。sPLA也与帕金森病、阿尔茨海默病和克罗恩病等神经炎症性疾病有关。
目的
本研究旨在探讨一种常见药用植物（Linn.）对人sPLA的抑制作用及其抗炎活性。
材料与方法
制备该植物的水提取物和不同有机溶剂提取物，并评估其对人滑液、人胸水以及（此处原文未完整给出两种磷脂酶具体名称）和毒液sPLA酶的抑制作用。
结果
在这些提取物中，该植物的乙醇提取物（EEBD）显示出最高的sPLA抑制作用，IC值范围为17.8至27.5μg。此外，使用2,2-二苯基-1-苦基肼自由基、硫代巴比妥酸和大鼠肝匀浆检测该植物提取物的抗氧化和脂质过氧化活性。EEBD的抗氧化活性或多或少与sPLA抑制作用成正比。最终，该提取物用于中和sPLA诱导的小鼠爪肿胀和间接溶血活性。EEBD在这两种情况下表现出相似的效力。
结论
研究结果表明，EEBD中的生物活性分子可能是观察到的对（此处原文未完整给出两种磷脂酶具体名称）和sPLA抑制作用及抗氧化活性的原因。
总结
本研究旨在探讨一种常见药用植物（Linn.）对人sPLA的抑制作用及其抗炎活性。EEBD：胀果甘草乙醇提取物，sPLA：分泌型磷脂酶A，HSF：人滑液，HPF：人胸水，VRV-PLA2-V：（此处原文未完整给出磷脂酶具体名称），NN-PLA-I：（此处原文未完整给出磷脂酶具体名称）
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