Department of Chemistry, University at Albany, 1400 Washington Avenue, Albany, NY 12222, USA.
Anal Bioanal Chem. 2011 Nov;401(8):2511-8. doi: 10.1007/s00216-011-5338-z. Epub 2011 Aug 26.
Raman spectroscopy was used to study trends in chemical composition of bones in a burial environment. A turkey bone was sectioned and buried for short intervals between 12 and 62 days. Buried sections were analyzed using Raman microspectroscopy with 785 nm excitation. The results indicate that chemical changes in bone due to soil bacteria are time-dependent. Spectroscopic trends within buried bone segments were correlated to burial duration. A preliminary model was constructed using peak integration of Raman bands. Data collected within buried bone segments fit very well in this model. The model constructed is sensitive to changes in bone composition in a scale of days. This study illustrates the great potential of Raman spectroscopy as a non-destructive method for estimating the burial duration of bone for forensic purposes.
拉曼光谱被用于研究埋葬环境中骨骼化学成分的变化趋势。将火鸡骨切成段，并在 12 至 62 天的短时间内进行埋葬。使用 785nm 激发的拉曼微光谱对埋藏的骨段进行分析。结果表明，土壤细菌引起的骨骼化学变化是时间依赖性的。埋藏骨段内的光谱趋势与埋藏时间有关。使用拉曼带的峰积分构建了初步模型。在这个模型中，收集到的埋藏骨段的数据拟合得非常好。所构建的模型对骨骼成分在几天的时间尺度上的变化非常敏感。这项研究说明了拉曼光谱作为一种非破坏性方法，在法医上估计骨骼埋葬时间具有巨大的潜力。
